Electrochemical immunosensor based on binary nanoparticles decorated rGO-TEPA as magnetic capture and Au@PtNPs as probe for CEA detection,Microchimica Acta

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Electrochemical immunosensor based on binary nanoparticles decorated rGO-TEPA as magnetic capture and Au@PtNPs as probe for CEA detection
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-29 , DOI: 10.1007/s00604-020-04559-2
Liangli Cao _{1,

2} , Haolin Xiao _{1,

3} , Cheng Fang ₁ , Feijun Zhao _{1,

3} , Zhencheng Chen _{1,

3}

Affiliation

Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab1), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab2) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL−1~200.0 ng mL−1) as well as low detection limit (1.42 pg mL−1). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis. Graphical Abstract Graphical Abstract

中文翻译：

基于二元纳米粒子修饰的rGO-TEPA作为磁捕获和Au@PtNPs作为CEA检测探针的电化学免疫传感器

以金和磁性纳米粒子共修饰的还原氧化石墨烯-四亚乙基五胺（rGO-TEPA/Au-MNPs）为磁性平台，用于捕获一抗（Ab1）、分离和预浓缩免疫复合物，成功开发了一种新型均相电化学免疫传感器。新制备的磁性 rGO-TEPA/Au-MNPs 与 MNPs 相比，由于 rGO-TEPA 表现出更好的稳定性和增强的导电性，并且由于 AuNPs 显示出更高的生物识别效率。此外，Au@PtNPs 被制备并用二抗 (Ab2) 修饰作为信号读出的有效信号探针。使用癌胚抗原（CEA）作为模型分析物，制备的免疫传感器表现出令人满意的特性，如高稳定性、良好的重复性和选择性、宽线性范围（5. 0 pg mL-1~200.0 ng mL-1) 以及低检测限 (1.42 pg mL-1)。将均相电化学免疫传感器应用于人血清中CEA的检测，发现与参考方法具有良好的相关性。因此，所提出的基于 rGO-TEPA/Au-MNPs 的同质免疫测定平台可能为生物标志物诊断开辟一条新途径。图形摘要图形摘要

更新日期：2020-09-29

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