Abstract
Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab1), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab2) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL−1~200.0 ng mL−1) as well as low detection limit (1.42 pg mL−1). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis.
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Funding
This research was supported in part by grants from the National Natural Science Foundation of China (61627807 and 81873913), Guangxi Key Research and Development Program (Guike AB20072003), and Natural Science Foundation of Guangxi (2018GXNSFDA281044), and also by China Postdoctoral Science Foundation (2019M653315), Guangxi Key Laboratory of Automatic Detecting Technology and Instruments (YQ20108) as well as Middle-aged and Young Teachers’ Basic Ability Promotion Project of Guangxi (2019KY0207).
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Cao, L., Xiao, H., Fang, C. et al. Electrochemical immunosensor based on binary nanoparticles decorated rGO-TEPA as magnetic capture and Au@PtNPs as probe for CEA detection. Microchim Acta 187, 584 (2020). https://doi.org/10.1007/s00604-020-04559-2
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DOI: https://doi.org/10.1007/s00604-020-04559-2