The toxic effects of the amorphous silica nanoparticles have not been thoroughly studied. Moreover, the majority of the in vivo investigations were performed using an inhalation exposure method. The current study aimed to explore the potential toxic effects of silica nanoparticles (SiNPs) after the treatment of adult male rats with two different concentrations (500 and 1000 ppm) via drinking water for 28 days. The genotoxicity, antioxidant status, and liver and kidney functions were assessed. Besides, histopathological and immunohistochemical evaluations were performed. The results showed a significant elevation in the malondialdehyde (MDA) level concurrent with a reduction in total reduced glutathione (GSH) concentration and catalase activity in the 1000-ppm SiNP-exposed rats as well as increase in ALT and AST activity confirmed by various histopathological alterations detected in liver. Also, in the 1000-ppm SiNP-exposed animals, there was an elevation in urea and creatinine levels confirmed by histopathological alterations detected in kidneys. Immunohistochemical findings in both liver and kidneys indicated strong expression of caspase-3 in the 1000-ppm SiNP-treated rats compared with the control and 500-ppm SiNP-treated groups. Such findings indicated that the 1000-ppm SiNPs exerted severe hepato-renal toxic impacts when compared with the control and 500-ppm SiNP-exposed rats.

无定形二氧化硅纳米粒子的毒性作用尚未得到彻底研究。此外，大多数体内研究是使用吸入暴露法进行的。目前的研究旨在探索二氧化硅纳米粒子 (SiNPs) 在通过饮用水处理两种不同浓度 (500 和 1000 ppm) 的成年雄性大鼠 28 天后的潜在毒性作用。评估了遗传毒性、抗氧化状态和肝肾功能。此外，还进行了组织病理学和免疫组织化学评估。结果显示，暴露于 1000 ppm SiNP 的大鼠的丙二醛 (MDA) 水平显着升高，同时总还原型谷胱甘肽 (GSH) 浓度和过氧化氢酶活性降低，以及各种组织病理学证实的 ALT 和 AST 活性增加。在肝脏中检测到改变。此外，在暴露于 1000 ppm SiNP 的动物中，肾脏中检测到的组织病理学改变证实了尿素和肌酐水平升高。肝脏和肾脏的免疫组织化学结果表明，与对照组和 500 ppm SiNP 治疗组相比，1000 ppm SiNP 治疗组中 caspase-3 的强表达。这些发现表明，与对照组和 500 ppm SiNP 暴露大鼠相比，1000 ppm SiNP 对肝肾毒性产生严重影响。