Objective The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. Results After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be detected while less than 10% of the same cells could be detected by flow cytometry (FCM) if spiked into blood. However, in combination with CD326 and CD45 immunomagnetic beads, detection rate of MCF7 cells in blood reached 57%. For circulating tumor cells, enrichment by CD326 and CD45 immunomagnetic beads improve the detection rate from nearly undetectable to more than 24.14%. Conclusions Live CTCs in peripheral blood can be effectively and sensitively detected by using a combination of immunomagnetic beads (CD45 and CD326) and flow cytometry.

中文翻译：

---

免疫磁珠和流式细胞术富集和检测循环肿瘤细胞

目的建立一种利用免疫磁珠和流式细胞术分析外周血循环肿瘤细胞（CTCs）的快速富集检测方法。结果与CD326-PE和CD45-APC抗体孵育后，在M-Buffer中可检测到超过60%的MCF7细胞，而如果掺入血液中，可通过流式细胞术(FCM)检测不到10%的相同细胞。而结合CD326和CD45免疫磁珠，血液中MCF7细胞的检出率达到57%。对于循环肿瘤细胞，CD326 和 CD45 免疫磁珠的富集将检测率从几乎检测不到提高到 24.14% 以上。