Phylogenetic diversity of culturable actinobacteria isolated from the intertidal regions of west coast of Maharashtra, India was studied using 16S rRNA gene sequencing. Total of 140 actinobacterial isolates were obtained, which belonged to 14 genera, 10 families and 65 putative species with Streptomyces being the most dominant (63%) genus followed by Nocardiopsis and Micromonospora. Isolates were screened for production of extracellular protease inhibitors (PI) against three pure proteases viz. chymotrypsin, trypsin, subtilisin and a crude extracellular protease from Pseudomonas aeruginosa. Eighty percent of the isolates showed PI activity against at least one of the four proteases, majority of these belonged to genus Streptomyces. Actinobacterial diversity from two sites Ade (17° 52' N, 73° 04' E) and Harnai (17° 48' N, 73° 05' E) with varying anthropological pressure showed that more putative species diversity was obtained from site with lower human intervention i.e. Ade (Shannon's H 3.45) than from Harnai (Shannon's H 2.83), a site with more human intervention. However, in Ade, percentage of isolates not showing PI activity against any of the proteases was close to 21% and that in Harnai was close to 9%. In other words, percentage of PI producers was lower at a site with lesser human intervention.

中文翻译：

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从印度马哈拉施特拉邦潮间带分离的产生蛋白酶抑制剂的可培养放线菌的多样性

使用 16S rRNA 基因测序研究了从印度马哈拉施特拉邦西海岸潮间带地区分离的可培养放线菌的系统发育多样性。共获得140株放线菌，属于14属10科65种，其中链霉菌属占优势（占63%），其次是诺卡氏菌属和小单孢菌属。筛选针对三种纯蛋白酶的细胞外蛋白酶抑制剂 (PI) 生产的分离物，即。糜蛋白酶、胰蛋白酶、枯草杆菌蛋白酶和来自铜绿假单胞菌的粗胞外蛋白酶。80% 的分离株显示出针对四种蛋白酶中至少一种的 PI 活性，其中大部分属于链霉菌属。来自 Ade（北纬 17° 52'，东经 73° 04'）和 Harnai（北纬 17° 48'，北纬 73° 05'）两个地点的放线菌多样性 E) 不同的人类学压力表明，与人类干预较多的 Harnai (Shannon's H 2.83) 相比，从人类干预较少的地点，即 Ade (Shannon's H 3.45) 获得了更多的假定物种多样性。然而，在 Ade 中，对任何蛋白酶不显示 PI 活性的分离株百分比接近 21%，而在 Harnai 中接近 9%。换句话说，在人为干预较少的地点，PI 生产者的百分比较低。对任何蛋白酶不显示 PI 活性的分离株百分比接近 21%，而在 Harnai 中接近 9%。换句话说，在人为干预较少的地点，PI 生产者的百分比较低。对任何蛋白酶不显示 PI 活性的分离株百分比接近 21%，而在 Harnai 中接近 9%。换句话说，在人为干预较少的地点，PI 生产者的百分比较低。