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Diversity of Culturable Actinobacteria Producing Protease Inhibitors Isolated from the Intertidal Zones of Maharashtra, India

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Abstract

Phylogenetic diversity of culturable actinobacteria isolated from the intertidal regions of west coast of Maharashtra, India was studied using 16S rRNA gene sequencing. Total of 140 actinobacterial isolates were obtained, which belonged to 14 genera, 10 families and 65 putative species with Streptomyces being the most dominant (63%) genus followed by Nocardiopsis and Micromonospora. Isolates were screened for production of extracellular protease inhibitors (PI) against three pure proteases viz. chymotrypsin, trypsin, subtilisin and a crude extracellular protease from Pseudomonas aeruginosa. Eighty percent of the isolates showed PI activity against at least one of the four proteases, majority of these belonged to genus Streptomyces. Actinobacterial diversity from two sites Ade (17° 52′ N, 73° 04′ E) and Harnai (17° 48′ N, 73° 05′ E) with varying anthropological pressure showed that more putative species diversity was obtained from site with lower human intervention i.e. Ade (Shannon’s H 3.45) than from Harnai (Shannon’s H 2.83), a site with more human intervention. However, in Ade, percentage of isolates not showing PI activity against any of the proteases was close to 21% and that in Harnai was close to 9%. In other words, percentage of PI producers was lower at a site with lesser human intervention.

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Data Availability

Sequences of 16S rRNA gene of studied isolates are submitted to GenBank NCBI under the accession numbers MN339687–MN339893. Data used for analysis are provided in Supplementary Table 1.

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Acknowledgements

Financial support for this project was provided by Rajiv Gandhi Science and Technology Commission under the Maharashtra Gene Bank Program. We thank Dr. Milind Watve for his critical comments and suggestions for this manuscript. Thanks are due to Dr. Neelesh Dahanukar for his help in construction of phylogenetic tree. We also thank Mr. Asim Auti for providing Pseudomonas aeruginosa culture and Mr. Chinmay Kulkarni for assisting in the fieldwork.

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Authors and Affiliations

  1. Department of Microbiology, M.E.S. Abasaheb Garware College, Karve Road, Pune, Maharashtra, 411004, India

    Neha A. Shintre & Neelima M. Deshpande

  2. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India

    Vaijayanti A. Tamhane

  3. Indian Institute of Science Education and Research, Pune (IISER-P), Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra, 411008, India

    Ulfat I. Baig & Anagha S. Pund

  4. Department of Microbiology, Haribhai V. Desai College of Commerce, Arts and Science, Pune, Maharashtra, 411002, India

    Rajashree B. Patwardhan

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  1. Neha A. Shintre
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Contributions

ND, VT, NS and RP conceived and designed the study. NS, UB, and AP performed the study. NS and VT analysed the data. NS, VT, and ND wrote the manuscript with inputs from other authors. All authors contributed to the proofreading of the manuscript.

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Correspondence to Neelima M. Deshpande.

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Shintre, N.A., Tamhane, V.A., Baig, U.I. et al. Diversity of Culturable Actinobacteria Producing Protease Inhibitors Isolated from the Intertidal Zones of Maharashtra, India. Curr Microbiol 77, 3555–3564 (2020). https://doi.org/10.1007/s00284-020-02174-1

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