Brucellosis is a zoonosis caused by bacteria of the Brucella genus. Any source of contamination that could be infectious must be monitored to reduce the risk of exposure to brucellosis, so the purpose of this work was to determine the presence of Brucella spp. on surface water and tilapia (Oreochromis niloticus) skin from a volcanic lake in Mexico. A seasonal sampling during 2016-2017 was carried out at fifteen specific sites for water sampling and five sites for the collection of tilapia fish. From all water and fish samples tested, we found only three isolates of Brucella species. We isolated and identified B. abortus from surface water through bacteriological and molecular techniques, and B. abortus and B. suis from the same tilapia skin sample. The isolated strains likely came from breeding animals that are common to the region, such as infected pigs or cattle with Brucella abortus or B. suis, respectively. A similar finding has not been reported in a water from volcanic lake or tilapia fish in Mexico. We concluded that B. abortus and B. suis are present on the surface water of the volcanic lake and tilapia skin as possible contaminants derived from biological material from cows and pigs carrying this bacterium.

中文翻译：

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墨西哥火山湖中两种布鲁氏菌的分离鉴定

布鲁氏菌病是由布鲁氏菌属细菌引起的人畜共患病。必须监测任何可能具有传染性的污染源，以降低接触布鲁氏菌病的风险，因此这项工作的目的是确定布鲁氏菌属的存在。在来自墨西哥火山湖的地表水和罗非鱼（Oreochromis niloticus）皮肤上。2016-2017 年期间，在 15 个特定水样采样点和 5 个罗非鱼采集点进行了季节性采样。从所有测试的水和鱼样本中，我们只发现了三种布鲁氏菌种分离物。我们通过细菌学和分子技术从地表水中分离并鉴定了流产双歧杆菌，并从同一罗非鱼皮肤样本中分离并鉴定了流产双歧杆菌和猪双歧杆菌。分离的菌株可能来自该地区常见的繁殖动物，例如分别感染流产布鲁氏菌或猪双歧杆菌的猪或牛。在墨西哥火山湖或罗非鱼的水中没有报道过类似的发现。我们得出结论，B. abortus 和 B. suis 存在于火山湖和罗非鱼皮肤的地表水中，它们可能是来自携带这种细菌的奶牛和猪的生物材料的污染物。