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Isolation and Identification of Two Brucella Species from a Volcanic Lake in Mexico

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Abstract

Brucellosis is a zoonosis caused by bacteria of the Brucella genus. Any source of contamination that could be infectious must be monitored to reduce the risk of exposure to brucellosis, so the purpose of this work was to determine the presence of Brucella spp. on surface water and tilapia (Oreochromis niloticus) skin from a volcanic lake in Mexico. A seasonal sampling during 2016–2017 was carried out at fifteen specific sites for water sampling and five sites for the collection of tilapia fish. From all water and fish samples tested, we found only three isolates of Brucella species. We isolated and identified B. abortus from surface water through bacteriological and molecular techniques, and B. abortus and B. suis from the same tilapia skin sample. The isolated strains likely came from breeding animals that are common to the region, such as infected pigs or cattle with Brucella abortus or B. suis, respectively. A similar finding has not been reported in a water from volcanic lake or tilapia fish in Mexico. We concluded that B. abortus and B. suis are present on the surface water of the volcanic lake and tilapia skin as possible contaminants derived from biological material from cows and pigs carrying this bacterium.

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Funding

This study was funded by Consejo Nacional de Ciencia y Tecnología, México (Grant Number INFR-2012–2:188090).

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Authors and Affiliations

  1. Posgrado en Ciencias Ambientales, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, 72570, Puebla, Mexico

    Lesset Del Consuelo Ramos-Ramírez & Elsa Iracena Castañeda-Roldán

  2. Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias Y Benemérita Universidad Autónoma de Puebla, 72570, Puebla, Puebla, Mexico

    Zeus Saldaña-Ahuactzi, Ygnacio Martínez-Laguna & Elsa Iracena Castañeda-Roldán

  3. Facultad de Ciencias Químicas, Departamento Bioquímica – Alimentos, Benemérita Universidad, 72570, Puebla, Puebla, Mexico

    Laura Morales-Lara

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  1. Lesset Del Consuelo Ramos-Ramírez
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  5. Elsa Iracena Castañeda-Roldán
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Contributions

LdCRR: Conceived and designed the analysis, collected the data, contributed data or analysis tools, performed the analysis, and wrote the paper. ZSA: Conceived and designed the analysis, collected the data, contributed data or analysis tools, performed the analysis, and wrote the paper. LML: Conceived and designed the analysis, collected the data, contributed data or analysis tools and article review. YML: Contributed data or analysis tools and article review. EICR: Conceived and designed the analysis, collected the data, contributed data or analysis tools, performed the analysis, and wrote the paper.

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Correspondence to Elsa Iracena Castañeda-Roldán.

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Ramos-Ramírez, L.D.C., Saldaña-Ahuactzi, Z., Morales-Lara, L. et al. Isolation and Identification of Two Brucella Species from a Volcanic Lake in Mexico. Curr Microbiol 77, 3565–3572 (2020). https://doi.org/10.1007/s00284-020-02184-z

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