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An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters
RSC Advances ( IF 3.9 ) Pub Date : 2020-9-24 , DOI: 10.1039/d0ra04609d Baozhu Zhang 1 , Chunying Wei 2
RSC Advances ( IF 3.9 ) Pub Date : 2020-9-24 , DOI: 10.1039/d0ra04609d Baozhu Zhang 1 , Chunying Wei 2
Affiliation
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A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs). Two DNA templates were designed as the functional structures consisting of the Cu/Ag NC-nucleation segment located at two termini or one terminus and the aptamer segment in the middle of a DNA template. Two darkish DNA-Cu/Ag NCs came close to each other when the aptamer combined with the target due to the conformational alteration of the aptamer structure, resulting in an increased fluorescence signal readout. Thrombin was sensitively determined as low as 1.6 nM in the range of 1.6–8.0 nM with a high selectivity. Finally, this sensor succeeded in detecting thrombin in a real fetal bovine serum.
中文翻译：
基于开启荧光 DNA 模板的 Cu/Ag 纳米团簇无标记检测凝血酶的适体传感器
基于由目标识别诱导的适体构象的改变和由于两个深色 DNA 模板铜/银纳米簇 (DNA-Cu/Ag NCs) 的接近而导致的开启荧光，构建了一种高度灵敏的凝血酶适体传感器。两个 DNA 模板被设计为由位于两个末端或一个末端的 Cu/Ag NC 成核片段和位于 DNA 模板中间的适体片段组成的功能结构。由于适体结构的构象改变，当适体与靶结合时，两个暗色 DNA-Cu/Ag NCs 彼此靠近，导致荧光信号读数增加。凝血酶在 1.6-8.0 nM 范围内被灵敏地测定为低至 1.6 nM，具有高选择性。最后，
更新日期：2020-09-24
中文翻译：
基于开启荧光 DNA 模板的 Cu/Ag 纳米团簇无标记检测凝血酶的适体传感器
基于由目标识别诱导的适体构象的改变和由于两个深色 DNA 模板铜/银纳米簇 (DNA-Cu/Ag NCs) 的接近而导致的开启荧光，构建了一种高度灵敏的凝血酶适体传感器。两个 DNA 模板被设计为由位于两个末端或一个末端的 Cu/Ag NC 成核片段和位于 DNA 模板中间的适体片段组成的功能结构。由于适体结构的构象改变，当适体与靶结合时，两个暗色 DNA-Cu/Ag NCs 彼此靠近，导致荧光信号读数增加。凝血酶在 1.6-8.0 nM 范围内被灵敏地测定为低至 1.6 nM，具有高选择性。最后，
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