Issue 58, 2020, Issue in Progress
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RSC Advances

An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters†

Baozhu Zhanga  and  Chunying Wei ORCID logo *b  

* Corresponding authors

a College of Chemistry and Chemical Engineering, Jinzhong University, Yuci 030619, P. R. China

b Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, P. R. China
E-mail: weichuny@sxu.edu.cn

Abstract

A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs). Two DNA templates were designed as the functional structures consisting of the Cu/Ag NC-nucleation segment located at two termini or one terminus and the aptamer segment in the middle of a DNA template. Two darkish DNA-Cu/Ag NCs came close to each other when the aptamer combined with the target due to the conformational alteration of the aptamer structure, resulting in an increased fluorescence signal readout. Thrombin was sensitively determined as low as 1.6 nM in the range of 1.6–8.0 nM with a high selectivity. Finally, this sensor succeeded in detecting thrombin in a real fetal bovine serum.

Graphical abstract: An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

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Article information

DOI
https://doi.org/10.1039/D0RA04609D
Article type
Paper
Submitted
25 May 2020
Accepted
09 Sep 2020
First published
24 Sep 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 35374-35380

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An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

B. Zhang and C. Wei, RSC Adv., 2020, 10, 35374 DOI: 10.1039/D0RA04609D

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