Abstract

We study the factors affecting the size of two-layer polyionic bienzyme SOD1/catalase conjugates: the nature and concentration of the buffer solution, the pH maintained during particle synthesis, and the initial molar ratio of the enzymes. The optimal conditions for obtaining conjugates of the minimum size are determined. The conjugates obtained under these conditions demonstrate the high storage stability of both enzymes.

中文翻译：

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超氧化物歧化酶1和最小尺寸的过氧化氢酶的两层聚离子双酶复合物的合成

摘要

我们研究了影响两层聚离子双酶SOD1 /过氧化氢酶结合物大小的因素：缓冲溶液的性质和浓度，颗粒合成过程中维持的pH值以及酶的初始摩尔比。确定获得最小尺寸的缀合物的最佳条件。在这些条件下获得的缀合物证明了两种酶的高储存稳定性。