Abstract
We study the factors affecting the size of two-layer polyionic bienzyme SOD1/catalase conjugates: the nature and concentration of the buffer solution, the pH maintained during particle synthesis, and the initial molar ratio of the enzymes. The optimal conditions for obtaining conjugates of the minimum size are determined. The conjugates obtained under these conditions demonstrate the high storage stability of both enzymes.
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Funding
This work was supported by the Russian Foundation for Basic Research (project nos. 17-54-33027 and 18-29-09154) and the state registration topic AAAA-A16-116052010081-5. The equipment purchased under the Development Program of Moscow State University was used.
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Translated by O. Zhukova
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Tagirova, M.A., Eremeev, N.L., Vaneev, A.N. et al. Synthesis of a Two-Layer Polyionic Bienzyme Complex of Superoxide Dismutase 1 and Catalase of the Smallest Size. Moscow Univ. Chem. Bull. 75, 238–242 (2020). https://doi.org/10.3103/S0027131420040082
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DOI: https://doi.org/10.3103/S0027131420040082