Biological sulphate reduction (BSR) has been identified as a promising alternative for treating acid mine drainage. In this study, the effect of pH, temperature, and hydraulic retention time (HRT) on BSR was investigated. The Box–Behnken design was used to matrix independent variables, namely pH (4–6), temperature (10–30 °C), and HRT (2–7 days) with the sulphate reduction efficiency and sulphate reduction rate as response variables. Experiments were conducted in packed bed reactors operating in a downflow mode. Response surface methodology was used to statistically analyse the data and to develop statistical models that can be used to fully understand the individual effects and the interactions between the independent variables. The analysis of variance results showed that the data fitted the quadratic models well as confirmed by a non-significant lack of fit. The temperature and HRT effect were significant (p 0.05).

中文翻译：

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使用响应面法评估 pH、温度和水力停留时间对生物硫酸盐还原的影响

生物硫酸盐还原 (BSR) 已被确定为处理酸性矿山排水的有前途的替代方法。在这项研究中，研究了 pH 值、温度和水力停留时间 (HRT) 对 BSR 的影响。Box-Behnken 设计用于矩阵自变量，即 pH (4-6)、温度 (10-30 °C) 和 HRT（2-7 天），其中硫酸盐还原效率和硫酸盐还原速率作为响应变量。实验在以下流模式运行的填充床反应器中进行。响应面方法用于对数据进行统计分析并开发可用于充分了解个体效应和自变量之间相互作用的统计模型。方差分析结果表明，数据符合二次模型，并通过不显着的拟合缺失得到证实。温度和 HRT 效应显着 (p = 0.05)。