Abstract

The role of the DnaKJE and Trigger Factor (TF) chaperones in folding and refolding of proteins in Bacillus subtilis is studied. Bacterial luciferases of Photobacterium leiognathi and Photorhabdus luminescens as protein substrates have been used. It is shown that DnaKJE takes part in folding but not in refolding of the thermal inactivated proteins. It is shown that TF takes part in the synthesis of thermolabile P. leiognathi luciferase, but significantly decreases the level of synthesis of thermostable P. luminescens luciferase.

中文翻译：

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DnaK伴侣参与但不参与枯草芽孢杆菌热灭活蛋白的折叠

摘要

研究了DnaKJE和触发因子（TF）分子伴侣在枯草芽孢杆菌中蛋白质折叠和重折叠中的作用。的细菌萤光素酶鳆发光和发光光已被用作蛋白质底物。结果表明，DnaKJE参与热灭活蛋白的折叠，但不参与其折叠。结果表明，TF参与了耐热不育的白僵菌荧光素酶的合成，但显着降低了耐热的发光假单胞菌荧光素酶的合成水平。