Abstract

Nonrandom distribution of meiotic recombination events along the chromosomes shapes the diversity of potential genetic combinations among the offspring. To redistribute the chromosomal regions involved in recombination events, it was proposed to use meiosis-specific genes of Spo11 proteins (generating double-strand DNA breaks) from phylogenetically different organisms. For these purposes, transgenic tomato plants expressing native SPO11 genes from yeast (Saccharomyces cerevisae) or Arabidopsis thaliana under the control of constitutive 35S CaMV promoter were constructed. Genetic analysis showed that expression of both target SPO11 genes partly disturbed the monogenic inheritance of marker Wv:wv alleles in chromosome 2 and suppressed the crossing over in the region between the wv and d genes. A stable negative correlation between the target gene expression levels and the decrease in the frequency of crossing over in the analyzed chromosomal region was found. The possible genetic mechanisms underlying the redistribution of crossovers along chromosome 2 resulting from the target SPO11 gene expression are discussed.

中文翻译：

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重组SPO11基因在番茄中的表达局部改变交叉

摘要

沿染色体的减数分裂重组事件的非随机分布决定了后代中潜在遗传组合的多样性。为了重新分布参与重组事件的染色体区域，有人建议使用来自系统发育不同生物的Spo11蛋白的减数分裂特异性基因（产生双链DNA断裂）。为了这些目的，构建了在组成型35S CaMV启动子的控制下表达来自酵母（酿酒酵母）或拟南芥的天然SPO11基因的转基因番茄植物。遗传分析表明，两个目标SPO11基因的表达部分打乱了标记Wv：wv的单基因遗传等位基因在2号染色体上，并抑制了wv和d基因之间区域的交叉。发现在分析的染色体区域中，目标基因表达水平与交叉频率降低之间存在稳定的负相关性。讨论了由目标SPO11基因表达导致的沿着2号染色体的交叉重新分布的潜在遗传机制。