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An electrochemical DNA biosensor based on nitrogen-doped graphene nanosheets decorated with gold nanoparticles for genetically modified maize detection
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-22 , DOI: 10.1007/s00604-020-04511-4 Fuli Liu 1 , Kai Li 2 , Yukun Zhang 1 , Jiaqi Ding 1 , Tingting Wen 1 , Xinwu Pei 3 , Yongliang Yan 3 , Wei Ji 1 , Jian Liu 1 , Xiao Zhang 1 , Liang Li 3
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-22 , DOI: 10.1007/s00604-020-04511-4 Fuli Liu 1 , Kai Li 2 , Yukun Zhang 1 , Jiaqi Ding 1 , Tingting Wen 1 , Xinwu Pei 3 , Yongliang Yan 3 , Wei Ji 1 , Jian Liu 1 , Xiao Zhang 1 , Liang Li 3
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A reliable electrochemical biosensor is reported based on nitrogen-doped graphene nanosheets and gold nanoparticle (Au/N-G) nanocomposites for the event-specific detection of GM maize MIR162. The differential pulse voltammetry response of methylene blue (MB) was chosen to monitor the target DNA hybridization event. Under the optimum conditions, the peak current increased linearly with the logarithm of the concentration of DNA in the range 1.0 × 10−14 to 1.0 × 10−8 M, and the detection limit was 2.52 × 10−15 M (S/N = 3). It is also demonstrated that the DNA biosensor has high selectivity, good stability, and fabrication reproducibility. The biosensor has been effectively applied to detect MIR162 in real samples, showing its potential as an effective tool for GM crop analysis. These results will contribute to the development of new portable transgenic detection systems. Graphical abstract . .
中文翻译：
基于金纳米粒子装饰的氮掺杂石墨烯纳米片的电化学 DNA 生物传感器用于转基因玉米检测
报道了一种基于氮掺杂石墨烯纳米片和金纳米颗粒 (Au/NG) 纳米复合材料的可靠电化学生物传感器，用于事件特异性检测转基因玉米 MIR162。选择亚甲蓝 (MB) 的微分脉冲伏安响应来监测目标 DNA 杂交事件。在最佳条件下，峰值电流在 1.0 × 10−14 至 1.0 × 10−8 M 范围内随 DNA 浓度的对数线性增加，检出限为 2.52 × 10−15 M（S/N = 3）。还证明了 DNA 生物传感器具有高选择性、良好的稳定性和制造重现性。该生物传感器已有效应用于检测实际样品中的 MIR162，显示其作为转基因作物分析的有效工具的潜力。这些结果将有助于开发新的便携式转基因检测系统。图形概要 。.
更新日期：2020-09-22
中文翻译：
基于金纳米粒子装饰的氮掺杂石墨烯纳米片的电化学 DNA 生物传感器用于转基因玉米检测
报道了一种基于氮掺杂石墨烯纳米片和金纳米颗粒 (Au/NG) 纳米复合材料的可靠电化学生物传感器，用于事件特异性检测转基因玉米 MIR162。选择亚甲蓝 (MB) 的微分脉冲伏安响应来监测目标 DNA 杂交事件。在最佳条件下，峰值电流在 1.0 × 10−14 至 1.0 × 10−8 M 范围内随 DNA 浓度的对数线性增加，检出限为 2.52 × 10−15 M（S/N = 3）。还证明了 DNA 生物传感器具有高选择性、良好的稳定性和制造重现性。该生物传感器已有效应用于检测实际样品中的 MIR162，显示其作为转基因作物分析的有效工具的潜力。这些结果将有助于开发新的便携式转基因检测系统。图形概要 。.
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