Abstract

Mutant strains of the halotolerant type-I methanotroph Methylotuvimicrobium alcaliphilum 20Z with an inactivated sps gene, which encodes sucrose phosphate synthase, and the deletion of a gene cluster for the synthesis and degradation of glycogen were obtained. Blockage of the synthesis of sucrose and glycogen increased the protein content in cells but slightly reduced the growth rate of the methanotroph grown on methane. It is shown that relatively stable methanotroph growth is possible without synthesis of the storage carbon compounds.

中文翻译：

---

糖原和蔗糖积累能力降低的I型元营养学的构建

摘要

所述耐盐-I型甲烷营养突变株Methylotuvimicrobium alcaliphilum与灭活20Z SPS得到基因，其编码蔗糖磷酸合成酶，和用于合成和糖原降解的基因簇的删除。蔗糖和糖原合成的阻断增加了细胞中的蛋白质含量，但略微降低了在甲烷上生长的甲烷营养菌的生长速率。结果表明，在不合成储存碳化合物的情况下，相对稳定的甲烷营养生长是可能的。