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Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation

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Abstract

Mutant strains of the halotolerant type-I methanotroph Methylotuvimicrobium alcaliphilum 20Z with an inactivated sps gene, which encodes sucrose phosphate synthase, and the deletion of a gene cluster for the synthesis and degradation of glycogen were obtained. Blockage of the synthesis of sucrose and glycogen increased the protein content in cells but slightly reduced the growth rate of the methanotroph grown on methane. It is shown that relatively stable methanotroph growth is possible without synthesis of the storage carbon compounds.

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Funding

This work was supported by the Ministry of Science and High Education of Russia, project no. 075-15-2019-1830, of the Federal Targeted Program. The unique identifier (UID) of the project is RFMEFI60719X0297.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research Federal Research Center, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    S. Yu. But & V. N. Khmelenina

  2. Winogradsky Institute of Microbiology, Biotechnology Research Center, Russian Academy of Sciences, Moscow, Russia

    S. N. Dedysh & N. V. Pimenov

  3. Bach Institute of Biochemistry, Biotechnology Research Center, Russian Academy of Sciences, Moscow, Russia

    V. O. Popov

Authors
  1. S. Yu. But
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  2. S. N. Dedysh
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  3. V. O. Popov
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  4. N. V. Pimenov
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  5. V. N. Khmelenina
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Contributions

All authors participated in the discussion of the results. All authors participated in the conceptualization of research tasks and the presentation of experimental results.

Corresponding author

Correspondence to V. N. Khmelenina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Bulaev

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But, S.Y., Dedysh, S.N., Popov, V.O. et al. Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation. Appl Biochem Microbiol 56, 538–543 (2020). https://doi.org/10.1134/S0003683820050063

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  • DOI: https://doi.org/10.1134/S0003683820050063

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