A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.

中文翻译：

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光驱动微型机器人进行单细胞弹性测量

引入了一种细胞弹性测量方法，该方法使用由全息光镊驱动的聚合物微型工具。用双光子聚合制备微工具。它们的形状使得细胞可以在任何横向方向上接近。在提出的情况下，用垂直方向的工具探测在垂直聚合物壁上生长的内皮细胞。特殊形状的微型工具的使用可防止靶细胞受到光捕获过程中可能发生的光损伤。工具的位置仅通过视频显微镜记录下来，并通过图像处理方法进行分析。我们将所得的杨氏模量值与其他方法获得的文献进行严格比较。光学镊子的应用将可用于细胞压痕测量的力范围扩展至fN范围。