Bioprocess and Biosystems Engineering ( IF 3.5 ) Pub Date : 2020-09-21 , DOI: 10.1007/s00449-020-02441-2 Alireza Habibi 1 , Salar Karami 2 , Kambiz Varmira 3 , Malihe Hadadi 1
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Abstract
Chitosan is commonly obtained from shrimp and crab shell chitin by deacetylation; however, such supplies appear limitation. An alternative source of chitosan is cell wall in certain fungi. In this study, chitosan production through submerged fermentation of Aspergillus terreus on apple waste extract as sole carbon source was investigated. Monod equation with a maximum specific growth rate of 0.083 h−1 and half-saturation constant of 6.67 w/v% was best described the kinetic of growth. Results of response surface methodology showed the highest chitosan to substrate yield of 49.32 mg gsubstrate−1, chitosan to fungal biomass yield of 140.9 mg gcell−1, and fungal biomass to substrate yield of 0.387 gcell gsubstrate−1 were simultaneously obtained at temperature 30.0 °C, initial pH 5.98, and ammonium nitrate concentration 5.0 g L−1. The chitosan produced at the optimum condition was characterized by FTIR, TGA, and DSC analysis, and degree of deacetylation was 88.2%.
Graphic abstract
中文翻译：
以苹果渣为唯一碳源的土曲霉生产壳聚糖关键参数优化[J].
摘要
壳聚糖通常是从虾和蟹壳几丁质中通过脱乙酰作用获得的；然而，这样的供应似乎有限。壳聚糖的另一种来源是某些真菌的细胞壁。在这项研究中，研究了土曲霉以苹果废料提取物作为唯一碳源的深层发酵生产壳聚糖。最大比增长率为 0.083 h -1和半饱和常数为 6.67 w/v% 的Monod 方程最能描述生长动力学。响应面方法的结果表明，壳聚糖对底物的产量最高为 49.32 mg g底物-1，壳聚糖对真菌生物量的产量为 140.9 mg g细胞-1，真菌生物量对底物的产量为 0.387 g细胞克基底-1在温度30.0℃，起始pH 5.98中同时获得，并且硝酸铵浓度5.0克大号-1。通过FTIR、TGA和DSC分析对最佳条件下生产的壳聚糖进行表征，脱乙酰度为88.2%。
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