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The Effect of Oncolytic Reovirus P-92 on Dendritic Cell Maturation and Generation of Tumor-Specific Lymphocytes in vitro
Cell and Tissue Biology Pub Date : 2020-02-20 , DOI: 10.1134/s1990519x19060099 A. O. Sitkovskaya , E. Yu. Zlatnik , I. V. Mezhevova , E. S. Bondarenko , S. A. Kolpakov , E. P. Kolpakova , I. A. Novikova
中文翻译:
溶瘤性呼肠孤病毒P-92对树突状细胞成熟和体外肿瘤特异性淋巴细胞生成的影响
更新日期:2020-02-20
Cell and Tissue Biology Pub Date : 2020-02-20 , DOI: 10.1134/s1990519x19060099 A. O. Sitkovskaya , E. Yu. Zlatnik , I. V. Mezhevova , E. S. Bondarenko , S. A. Kolpakov , E. P. Kolpakova , I. A. Novikova
Abstract
Utilization of oncolytic viruses (OVs) is a promising approach to inhibit the tumor growth. OVs exhibit both direct antitumor and indirect immunostimulatory effects. It is known that the recognition of tumor antigen by cells of the immune system is disturbed at cancer pathologies. Stimulation of dendritic cells (DCs) with tumor antigens together with viruses may enhance the generation of antigen-specific lymphocytes against tumor cells, which in turn can be an effective tool for antitumor cell technologies. The aim of this study was to clarify the possibility of using reovirus P-92 for the generation of tumor-specific T-lymphocytes in vitro. We found that the differentiation of DCs in the presence of reovirus P-92 increased the yield of mature DCs. In addition, DCs with loaded HeLa antigens after cocultivation with lymphocytes and HeLa cells did not demonstrate an active cytolytic activity. Only a cytostatic effect was observed, unlike in the case of samples with the added reovirus P-92. Thus, the antigen, which is HeLa cells cultured with reovirus P-92 under the conditions we described, can be proposed as an effective experimental product for the cellular cancer immunotherapy.中文翻译:
溶瘤性呼肠孤病毒P-92对树突状细胞成熟和体外肿瘤特异性淋巴细胞生成的影响