Abstract

Utilization of oncolytic viruses (OVs) is a promising approach to inhibit the tumor growth. OVs exhibit both direct antitumor and indirect immunostimulatory effects. It is known that the recognition of tumor antigen by cells of the immune system is disturbed at cancer pathologies. Stimulation of dendritic cells (DCs) with tumor antigens together with viruses may enhance the generation of antigen-specific lymphocytes against tumor cells, which in turn can be an effective tool for antitumor cell technologies. The aim of this study was to clarify the possibility of using reovirus P-92 for the generation of tumor-specific T-lymphocytes in vitro. We found that the differentiation of DCs in the presence of reovirus P-92 increased the yield of mature DCs. In addition, DCs with loaded HeLa antigens after cocultivation with lymphocytes and HeLa cells did not demonstrate an active cytolytic activity. Only a cytostatic effect was observed, unlike in the case of samples with the added reovirus P-92. Thus, the antigen, which is HeLa cells cultured with reovirus P-92 under the conditions we described, can be proposed as an effective experimental product for the cellular cancer immunotherapy.

中文翻译：

---

溶瘤性呼肠孤病毒P-92对树突状细胞成熟和体外肿瘤特异性淋巴细胞生成的影响

摘要

利用溶瘤病毒（OVs）是抑制肿瘤生长的一种有前途的方法。OVs具有直接的抗肿瘤作用和间接的免疫刺激作用。已知免疫系统细胞对肿瘤抗原的识别在癌症病理学上受到干扰。用肿瘤抗原和病毒一起刺激树突状细胞（DC）可以增强针对肿瘤细胞的抗原特异性淋巴细胞的生成，这反过来又可以成为抗肿瘤细胞技术的有效工具。这项研究的目的是阐明使用呼肠孤病毒P-92在体外产生肿瘤特异性T淋巴细胞的可能性。我们发现在呼肠孤病毒P-92存在下DC的分化增加了成熟DC的产量。此外，与淋巴细胞和HeLa细胞共培养后，带有负载HeLa抗原的DC并未表现出活性的细胞溶解活性。与添加呼肠孤病毒P-92的样品不同，仅观察到了细胞抑制作用。因此，可以将抗原（在我们描述的条件下用呼肠孤病毒P-92培养的HeLa细胞）建议为细胞癌免疫疗法的有效实验产品。