Objective

Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro.

Method

Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively.

Result

The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells.

Conclusion

Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.

中文翻译：

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沉默环状 RNA hsa_circ_009755 促进口腔鳞状细胞癌的生长和转移。

目标

口腔鳞状细胞癌 (OSCC) 是一种常见且猖獗的头颈部恶性肿瘤。然而，其发病机制尚不清楚。在这项研究中，我们研究了环状 RNA hsa_circ_009755 对体外 OSCC 细胞增殖、迁移、侵袭和凋亡的影响。

方法

选取 8 对 OSCC 和正常邻近组织，通过高通量测序检测 circRNA 的差异表达。circRNA hsa_circ_009755 的表达是通过定量实时聚合酶链反应在 OSCC 组织和细胞系中确定的。CCK-8、伤口愈合、Transwell 和流式细胞术测定分别用于确定 OSCC 细胞增殖、迁移、侵袭和凋亡。

结果

hsa_circ_009755 在 OSCC 组织和三种 OSCC 细胞系中的表达较低。沉默 hsa_circ_009755 可显着增强 OSCC 细胞的增殖、迁移和侵袭并抑制其凋亡。

结论

因此，hsa_circ_009755 可能在 OSCC 的肿瘤发生中很重要。