Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma

Highlights

• Hsa_circ_009755 is downregulated in oral squamous cell carcinoma.

• Silencing hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma.

• Hsa_circ_009755 acts as a potential biomarker for OSCC.

Abstract

Objective

Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro.

Method

Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively.

Result

The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells.

Conclusion

Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.