The process of genetically programmed cell death, or apoptosis, plays a crucialrolein cellular homeostasis and gene expression. Disruption of apoptosis may lead to aberrant immune responses, cancer, and neurodegenerative diseases. Single nucleotide polymorphisms (SNPs) present in various microRNA (miRNA) genes and targets being an alteration of miRNA activity resulting in human diseases. Evidence reported that SNPs increase/decrease the effectiveness of the interaction between miRNAs and their target genes associated with diseases. The primary purpose of this study is not only to identify miRSNPs on the CASP7 gene (caspase-7) and SNPs in miRNA genes targeting 3′UTR but also to evaluate the effect of thesegene variations in apoptosis and their associated diseases. We detected 120 miRNAs binding sites and 27 different SNPs in binding sites of miRNA in 3′UTR of the CASP7 gene by ten different online softwares. Interestingly, miR-371b-5p’s binding site on CASP7 has an SNP (rs576198588, G/T) on CASP7 3′UTR, and its genomic sequence has an SNP (rs751339395, G/T) at the same nucleotide with rs576198588. Similarly, two other SNPs (rs774879764, C/G rs750389063, C/T) were identified at the first position binding site of miR-371b-5p. Here, miRSNP (rs576198588) at CASP7 3′UTR and SNP (rs751339395) at miR-371b-5p genomic sequence cross-matches at the same site of binding region. Besides, miR-371b-5p targets many apoptosis-related genes (HIP1, TRIAP1, GSKIP, NIN, DAP, CAAP1, XIAP, TMBIM1, TMBIM4, TNFRSF10A, RAD21, AKT1, BAG1, BAG4) even though it had no apoptosis correlated interaction demonstrated formerly. It assures that CASP7 could have a significant consequence on apoptosis through different pathways. Henceforth, this study was representing and signifying an influential connotation among miR-371b-5p and apoptosis via computational exploration and recommended to have better insight.

基因程序性细胞死亡或细胞凋亡的过程在细胞稳态和基因表达中发挥着至关重要的作用。细胞凋亡的破坏可能导致异常的免疫反应、癌症和神经退行性疾病。单核苷酸多态性 (SNP) 存在于各种 microRNA (miRNA) 基因中，其靶标是 miRNA 活性的改变，从而导致人类疾病。有证据表明，SNP 会增加/降低 miRNA 与其与疾病相关的靶基因之间相互作用的有效性。本研究的主要目的不仅是鉴定CASP7基因 (caspase-7) 上的 mirRSNP 和针对 3'UTR 的 miRNA 基因中的 S​​NP，而且还评估这些基因变异对细胞凋亡及其相关疾病的影响。我们通过10个不同的在线软件检测了CASP7基因3'UTR中miRNA结合位点的120个miRNA结合位点和27个不同的SNP。有趣的是，miR-371b-5p在CASP7上的结合位点在CASP7 3'UTR上有一个SNP（rs576198588，G/T），其基因组序列在与rs576198588相同的核苷酸处有一个SNP（rs751339395，G/T）。类似地，在 miR-371b-5p 的第一个位置结合位点处鉴定了另外两个 SNP（rs774879764、C/G rs750389063、C/T）。此处， CASP7 3'UTR 处的 miRSNP (rs576198588) 和 miR-371b-5p 基因组序列处的 SNP (rs751339395) 在结合区的同一位点交叉匹配。此外，miR-371b-5p 靶向许多凋亡相关基因（ HIP1、TRIAP1、GSKIP、NIN、DAP、CAAP1、XIAP、TMBIM1、TMBIM4、TNFRSF10A、RAD21、AKT1、BAG1、BAG4 ），尽管它没有与凋亡相关的相互作用以前演示过。 它确保CASP7可以通过不同途径对细胞凋亡产生重大影响。此后，这项研究通过计算探索代表并表明了 miR-371b-5p 与细胞凋亡之间的影响力内涵，并建议获得更好的见解。