Although micropropagation in temporary immersion systems might increase plant growth and multiplication, it can also cause specific problems such as hyperhydricity and losses by contamination. A new commercial temporary immersion bioreactor, SETIS™, was used to micropropagate two Tunisian pear cultivars, ‘Arbi’ and ‘Mahdia 6’. The latter cultivar was endogenously contaminated by Sphingomonas. Hyperhydricity was inevitable when 5 µM benzyladenine was applied. However, the symptoms could be reduced by lowering the immersion frequency to 3 times per day. Applying 5 µM meta–Methoxy topolin riboside (MemTR) or meta–Topolin riboside (mTR) completely inhibited hyperhydric shoot formation. Moreover, the addition of Plant Preservative Mixture was effective to control Sphingomonas and allowed the plants to proliferate. For both pear cultivars, the highest number of shoots per explant was induced by 5 µM MemTR, whereas the highest leaf area was obtained with 5 µM mTR. The longest shoots were obtained with 5 µM mTR for ‘Arbi’ and 5 µM MemTR for ‘Mahdia 6’.

尽管临时浸没系统中的微繁殖可能会增加植物的生长和繁殖，但也会引起特定的问题，例如高水度和污染造成的损失。一种新型的商用临时浸入式生物反应器SETIS™用于微传播两个突尼斯梨品种'Arbi'和'Mahdia 6'。后者品种被鞘氨醇单胞菌内源性污染。当使用5 µM苄基腺嘌呤时，过高的水分不可避免。但是，可以通过将浸入频率降低到每天3次来减轻症状。应用5 µM间-甲氧基拓扑素核苷（Me m TR）或间-Topolin核苷（mTR）完全抑制高水生芽的形成。此外，添加植物防腐剂混合物可有效控制鞘氨醇单胞菌并使植物增殖。对于这两种梨品种，每个外植芽数最高诱导5μM我米TR，而用5μM获得了最高叶面积米TR。最长的芽用“ Arbi”用5 µM m TR和“ Mahdia 6”用5 µM Me m TR获得。