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Effects of meta–topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis

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Abstract

Although micropropagation in temporary immersion systems might increase plant growth and multiplication, it can also cause specific problems such as hyperhydricity and losses by contamination. A new commercial temporary immersion bioreactor, SETIS™, was used to micropropagate two Tunisian pear cultivars, ‘Arbi’ and ‘Mahdia 6’. The latter cultivar was endogenously contaminated by Sphingomonas. Hyperhydricity was inevitable when 5 µM benzyladenine was applied. However, the symptoms could be reduced by lowering the immersion frequency to 3 times per day. Applying 5 µM meta–Methoxy topolin riboside (MemTR) or meta–Topolin riboside (mTR) completely inhibited hyperhydric shoot formation. Moreover, the addition of Plant Preservative Mixture was effective to control Sphingomonas and allowed the plants to proliferate. For both pear cultivars, the highest number of shoots per explant was induced by 5 µM MemTR, whereas the highest leaf area was obtained with 5 µM mTR. The longest shoots were obtained with 5 µM mTR for ‘Arbi’ and 5 µM MemTR for ‘Mahdia 6’.

Key message

Methoxy topoline-riboside (MemTR) and meta-topoline-riboside (mTR) were used as alternatives to benzyladenine to prevent hyperhydricity during the micropropagation of pears in a new temporary immersion system. These cytokinins also increased the number of good quality shoots, characterized by large leaves and longer shoots.

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Abbreviations

BA:

N6-benzyladenine

Kin:

Kinetin

MemTR:

meta–Methoxy topolin riboside

mT:

meta–Topolin

mTR:

meta–Topolin riboside

PPM:

Plant preservative mixture

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Authors and Affiliations

  1. Research Unit on Agrobiodiversity (UR13AGR05), Department of Horticultural Sciences, Higher Agronomic Institute, IRESA-University of Sousse, Chott-Mariem, 4042, Sousse, Tunisia

    Mariem Lotfi & Messaoud Mars

  2. Regional Research Centre on Horticulture and Organic Agriculture (CRRHAB), IRESA-University of Sousse, Chott-Mariem, 4042, Sousse, Tunisia

    Chokri Bayoudh

  3. Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

    Mariem Lotfi & Stefaan Werbrouck

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  1. Mariem Lotfi
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  2. Chokri Bayoudh
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  3. Stefaan Werbrouck
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  4. Messaoud Mars
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Correspondence to Stefaan Werbrouck.

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Communicated by Barbara Mary Doyle Prestwich.

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Lotfi, M., Bayoudh, C., Werbrouck, S. et al. Effects of meta–topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis. Plant Cell Tiss Organ Cult 143, 499–505 (2020). https://doi.org/10.1007/s11240-020-01935-x

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  • DOI: https://doi.org/10.1007/s11240-020-01935-x

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