A fluorometric method based on molecularly imprinted upconversion fluorescence test strip was developed for the determination of tyramine. It exploited the green fluorescence of upconversion nanoparticles (UCNPs) and the specific recognition property of molecularly imprinted polymers (MIPs). UCNPs were attached to filter paper with glue, and MIPs were prepared via in situ polymerization on the surface of UCNPs by using tyramine as template, methacrylic acid as functional monomer, and ethylene glycol dimethacrylate as cross-linker. The green fluorescence of the test strip, with excitation/emission wavelength 980/550 nm, was enhanced by tyramine. The test strip was suitable for the determination of tyramine in the linear range 1.0–100.0 mg L−1, and a relatively low limit of detection (0.2 mg L−1) was achieved. The test strip also worked well for the quantitation of tyramine in spiked red wine and mature vinegar. Recoveries are ranged from 84.9 to 99.9%. The relative standard deviations are below 5.6%. Graphical abstract Graphical abstract

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分子印迹上转换荧光试纸荧光法测定酪胺

建立了基于分子印迹上转换荧光试纸条的荧光法测定酪胺。它利用了上转换纳米粒子 (UCNPs) 的绿色荧光和分子印迹聚合物 (MIPs) 的特异性识别特性。UCNPs用胶黏附在滤纸上，以酪胺为模板剂，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，在UCNPs表面原位聚合制备MIPs。酪胺增强了测试条的绿色荧光，激发/发射波长为 980/550 nm。该试纸适用于线性范围 1.0–100.0 mg L-1 内酪胺的测定，并实现了相对较低的检测限（0.2 mg L-1）。该试纸也适用于加标红酒和陈醋中酪胺的定量。回收率从 84.9% 到 99.9% 不等。相对标准偏差低于 5.6%。图形摘要图形摘要