The work presents a novel glucose oxidase@Cu-hemin metal-organic frameworks (GOD@ Cu-hemin MOFs) with a ball-flower structure as bienzymatic catalysts for detection of glucose. The GOD@Cu-hemin MOFs exhibits great stability as compared with free horseradish peroxidase and GOD toward harsh conditions because the ball-flower-like shell of Cu-hemin MOF effectively protects from GOD. Thus, the GOD@Cu-Hemin MOFs can be used in external harsh conditions such as high temperature and acid/base. The GOD@Cu-hemin MOFs is capable of sensitive and selective detection of glucose via peroxidase-like of Cu-hemin MOFs and GOD by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. Under the existence of glucose, O₂ is reduced into H₂O₂ via GOD@Cu-hemin MOFs. The produced H₂O₂ as well as Cu-hemin MOFs oxidize TMB into blue oxTMB which shows UV–Vis absorbance at 652. The absorption intensity of oxTMB linearly increases with the increasing concentration of glucose from 0.01 to 1.0 mM with detection limit of 2.8 μM. An integrated agarose hydrogel film (Aga/GOD@Cu-hemin MOF/TMB) sensor is rationally designed for colorimetric detection of glucose. The sensor displays a response range of 30 μM-0.8 mM with a detection limit of 0.01 mM. The result indicates that the Cu-hemin MOFs are an ideal carrier for the encapsulation of enzymes.

这项工作提出了一种具有球形花结构的新型葡萄糖氧化酶@铜-hemin金属有机框架（GOD @铜-hemin MOFs），作为检测葡萄糖的双酶催化剂。与游离辣根过氧化物酶和GOD相比，GOD @ Cu-hemin MOF在苛刻条件下表现出出色的稳定性，因为Cu-hemin MOF的球形花状外壳有效地保护了GOD。因此，GOD @ Cu-Hemin MOF可以在外部苛刻条件下使用，例如高温和酸/碱。通过使用3,3'，5,5'-四甲基联苯胺（TMB）作为底物，通过过氧化酶样的Cu-hemin MOF和GOD，GOD @ Cu-hemin MOF能够灵敏和选择性地检测葡萄糖。在葡萄糖存在下，O ₂还原为H ₂ O ₂通过GOD @ Cu-hemin MOF。产生的H ₂ O ₂和Cu-hemin MOF将TMB氧化为蓝色oxTMB，在652处显示UV-Vis吸光度。oxTMB的吸收强度随着葡萄糖浓度从0.01到1.0 mM线性增加，检测限为2.8微米 集成的琼脂糖水凝胶薄膜（Aga / GOD @ Cu-hemin MOF / TMB）传感器经过合理设计，可用于葡萄糖的比色检测。传感器显示的响应范围为30μM-0.8mM，检测极限为0.01 mM。结果表明，Cu-hemin MOF是酶包封的理想载体。