Glucose oxidase@Cu-hemin metal-organic framework for colorimetric analysis of glucose

https://doi.org/10.1016/j.msec.2020.111511Get rights and content

Highlights

  • A GOD@Cu-hemin MOFs was prepared by biomimetic mineralization strategy.

  • The GOD@Cu-hemin MOFs present ball-flower structure with peroxidase-like catalytic activity.

  • The GOD@Cu-hemin MOFs exhibited good catalytic activity and chemical stability toward harsh conditions.

  • An integrated Aga/GOD@Cu-hemin MOF/TMB film was designed for colorimetric detection of glucose.

  • The sensor displayed a good performance.

Abstract

The work presents a novel glucose oxidase@Cu-hemin metal-organic frameworks (GOD@ Cu-hemin MOFs) with a ball-flower structure as bienzymatic catalysts for detection of glucose. The GOD@Cu-hemin MOFs exhibits great stability as compared with free horseradish peroxidase and GOD toward harsh conditions because the ball-flower-like shell of Cu-hemin MOF effectively protects from GOD. Thus, the GOD@Cu-Hemin MOFs can be used in external harsh conditions such as high temperature and acid/base. The GOD@Cu-hemin MOFs is capable of sensitive and selective detection of glucose via peroxidase-like of Cu-hemin MOFs and GOD by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. Under the existence of glucose, O2 is reduced into H2O2 via GOD@Cu-hemin MOFs. The produced H2O2 as well as Cu-hemin MOFs oxidize TMB into blue oxTMB which shows UV–Vis absorbance at 652. The absorption intensity of oxTMB linearly increases with the increasing concentration of glucose from 0.01 to 1.0 mM with detection limit of 2.8 μM. An integrated agarose hydrogel film (Aga/GOD@Cu-hemin MOF/TMB) sensor is rationally designed for colorimetric detection of glucose. The sensor displays a response range of 30 μM-0.8 mM with a detection limit of 0.01 mM. The result indicates that the Cu-hemin MOFs are an ideal carrier for the encapsulation of enzymes.

Keywords

Metal-organic frameworks
Agarose hydrogel film
Glucose oxidase
Hemin
Glucose

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