Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using dual-color SiO2@QD nanotags,Microchimica Acta

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Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using dual-color SiO2@QD nanotags
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-17 , DOI: 10.1007/s00604-020-04555-6
Xingsheng Yang _{1,

2} , Xiaoxian Liu _{1,

2} , Bing Gu _{3,

4} , Haifeng Liu _{1,

2} , Rui Xiao ₂ , Chongwen Wang _{1,

2,

3} , Shengqi Wang ₂

Affiliation

An on-site detection strategy is reported based on dual-color SiO2@quantum dot (QD)–integrated lateral flow immunoassay (LFA) strip to realize the quantitative and simultaneous detection of C-reactive protein (CRP) and procalcitonin (PCT) in serum. The dual-color SiO2@QD nanotags with monodispersity and excellent luminescence were synthesized using polyethyleneimine-mediated electrostatic adsorption of dense red CdSe/ZnS-COOH (excitation/emission 365/625 nm) or green CdSe/ZnS-COOH (excitation/emission 365/525 nm) QDs on the surface of 180 nm SiO2 spheres and were conjugated with anti-PCT and anti-CRP monoclonal antibodies, as stable and fluorescent-enhanced QD nanotags in the LFA system. The use of SiO2@QDs with two different fluorescent signals caused the sensitivity and specificity of the multiplex LFA system. As a result, the proposed assay provided a wide logarithmic determination range with a CRP quantitative range of 0.5–103 ng/mL and PCT quantitative range of 0.05–103 ng/mL. The limits of detection (LODs) of CRP and PCT reached 0.5 and 0.05 ng/mL, respectively. The SiO2@QD-based LFA showed great potential as rapid detection tool for the simultaneous monitoring of CRP and PCT in serum sample.

中文翻译：

使用双色 SiO2@QD 纳米标签通过荧光侧流免疫分析定量和同时检测两种炎症生物标志物

报道了一种基于双色SiO2@量子点（QD）-集成横向流动免疫分析（LFA）条的现场检测策略，实现了C反应蛋白（CRP）和降钙素原（PCT）的定量和同时检测。血清。使用聚乙烯亚胺介导的致密红色 CdSe/ZnS-COOH（激发/发射 365/625 nm）或绿色 CdSe/ZnS-COOH（激发/发射 365 /525 nm) 位于 180 nm SiO2 球体表面的 QD，并与抗 PCT 和抗 CRP 单克隆抗体结合，作为 LFA 系统中稳定且荧光增强的 QD 纳米标签。使用具有两种不同荧光信号的 SiO2@QD 导致了多重 LFA 系统的灵敏度和特异性。因此，提议的测定提供了一个宽的对数测定范围，CRP 定量范围为 0.5-103 ng/mL，PCT 定量范围为 0.05-103 ng/mL。CRP 和 PCT 的检测限 (LOD) 分别达到 0.5 和 0.05 ng/mL。基于 SiO2@QD 的 LFA 作为同时监测血清样品中 CRP 和 PCT 的快速检测工具显示出巨大的潜力。

更新日期：2020-09-17

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