Background

Systemic inflammation has been implicated in the pathogenesis of moyamoya disease (MMD). Sortilin is a critical regulator of proinflammatory cytokine secretion in several cell types. The present study investigated the association between circulating sortilin and proinflammatory cytokine levels and the occurrence of MMD.

Methods

Forty-two MMD cases and 76 age- and sex-matched controls were enrolled in this study between January 2018 and June 2019 at the Affiliated Hospital of Jining Medical University. The demographic and clinical characteristics were evaluated, and the circulating serum and cerebrospinal fluid (CSF) levels of sortilin, sortilin-related receptor with A-type repeats (SorLA), and proinflammatory cytokines including C-reactive protein (CRP), interleukin (IL)-6, interferon (IFN)-γ were measured by enzyme-linked immunosorbent assay. Linear regression and correlation analyses were used to estimate the associations between sortilin, SorLA, and proinflammatory cytokine levels.

Results

MMD patients had higher serum levels of sortilin (P = 0.012), CRP (P = 0.013), IL-6 (P = 0.004), and IFN-γ (P = 0.033) than healthy controls. In MMD patients, serum sortilin was positively correlated with serum proinflammatory cytokines (CRP: r = 0.459, P = 0.0022; IL-6: r = 0.445, P = 0.0032; and IFN-γ: r = 0.448, P = 0.0029) and CSF sortilin (r = 0.440, P = 0.0035); the latter was positively correlated with CSF levels of CRP (r = 0.542, P = 0.0002), IL-6 (r = 0.440, P = 0.0036), and IFN-γ (r = 0.443, P = 0.0033).

Conclusions

Elevated sortilin level is associated MMD onset and may be a clinically useful biomarker along with proinflammatory cytokine levels.

中文翻译：

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循环分拣蛋白水平与烟雾病患者的炎症有关。

背景

全身性炎症与烟雾病 (MMD) 的发病机制有关。分拣蛋白是多种细胞类型中促炎细胞因子分泌的关键调节剂。本研究调查了循环分拣蛋白和促炎细胞因子水平与 MMD 发生之间的关联。

方法

2018 年 1 月至 2019 年 6 月在济宁医科大学附属医院的 42 例 MMD 病例和 76 例年龄和性别匹配的对照组参加了这项研究。评估了人口统计学和临床​​特征，循环血清和脑脊液 (CSF) 中分拣蛋白、带有 A 型重复序列的分拣蛋白相关受体 (SorLA) 和促炎细胞因子包括 C 反应蛋白 (CRP)、白细胞介素 (IL) 的水平)-6、干扰素(IFN)-γ通过酶联免疫吸附测定法测定。使用线性回归和相关分析来估计分拣蛋白、SorLA 和促炎细胞因子水平之间的关联。

结果

MMD 患者的分拣蛋白 (P = 0.012)、CRP (P = 0.013)、IL-6 (P = 0.004) 和 IFN-γ (P = 0.033) 的血清水平高于健康对照。在 MMD 患者中，血清分拣蛋白与血清促炎细胞因子呈正相关（CRP：r = 0.459，P = 0.0022；IL-6：r = 0.445，P = 0.0032；和 IFN-γ：r = 0.448，P = 0.0029）和脑脊液分拣蛋白 (r = 0.440, P = 0.0035); 后者与 CRP（r = 0.542，P = 0.0002）、IL-6（r = 0.440，P = 0.0036）和 IFN-γ（r = 0.443，P = 0.0033）的 CSF 水平呈正相关。

结论

升高的分拣蛋白水平与 MMD 发病相关，并且可能与促炎细胞因子水平一起成为临床上有用的生物标志物。