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Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western Himalaya.
Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2020-09-16 , DOI: 10.1007/s10482-020-01471-w
Rishabh Kaushik 1 , Meesha Sharma 1 , Kumar Gaurav 2 , U Jagadeeshwari 3 , A Shabbir 2 , Ch Sasikala 3 , Ch V Ramana 2 , Maharaj K Pandit 1
Affiliation  

A novel strain of Planctomycetes, designated JC670T, was isolated from a high altitude (~ 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding. Strain JC670T was found to grow well at pH 7.0 and pH 8.0 and to tolerate up to 2% NaCl (w/v). MK6 was the only respiratory quinone identified. The major fatty acids of strain JC670T were identified as C18:1ω9c, C18:0 and C16:0, and phosphatidylcholine, two unidentified phospholipids and six unidentified lipids are present as the polar lipids. The polyamines putrescine and sym-homospermidine were detected. Strain JC670T shows high 16S rRNA gene sequence identity (95.4%) with Paludisphaera borealis PX4T. The draft genome size of strain JC670T is 7.97 Mb, with G + C content of 70.4 mol%. Based on phylogenetic analyses with the sequences of ninety-two core genes, low dDDH value (20.6%), low gANI (76.8%) and low AAI (69.1%) results, differential chemotaxonomic and physiological properties, strain JC670T (= KCTC 72850T = NBRC 114339T) is recognised as the type strain of a new species of the genus Paludisphaera, for which we propose the name Paludisphaera soli sp. nov.



中文翻译:

草 十一月,是从喜马拉雅西部的高海拔土壤中分离出来的同工科的新成员。

浮霉菌门的新菌株,称为JC670 Ť,是从高海拔分离(〜2900米海拔)在西方喜马拉雅从格尔瓦区域采集的土壤样品。观察到该菌株的菌落为浅粉红色,具有球形至椭圆形的细胞,其颅状结构分布在整个细胞表面。细胞通过出芽分裂。发现菌株JC670 T在pH 7.0和pH 8.0时生长良好,并耐受高达2%的NaCl(w / v)。MK6是唯一鉴定出的呼吸醌。菌株JC670 T的主要脂肪酸鉴定为C 18: 1ω9c,C 18:0和C 16:0和磷脂酰胆碱,两种未鉴定的磷脂和六种未鉴定的脂质作为极性脂质存在。检测到多胺腐胺和顺高嘧啶。菌株JC670 T北极孢PX4 T表现出很高的16S rRNA基因序列同一性(95.4%)菌株JC670 T的基因组草图大小为7.97 Mb,G + C含量为70.4 mol%。根据对92个核心基因序列的系统发育分析,得出d DDH值低(20.6%),gANI值低(76.8%)和AAI值低(69.1%),化学分类和生理特性差异,菌株JC670 T(= KCTC) 72850 = NBRC 114339)被认为是Paludisphaera属新种的类型菌株 为此我们建议使用 Paludisphaera soli sp。十一月

更新日期:2020-09-16
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