Rapid and sensitive fluorescence and smartphone dual-mode detection of dopamine based on nitrogen-boron co-doped carbon quantum dots,Microchimica Acta

当前位置： X-MOL 学术 › Microchim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Rapid and sensitive fluorescence and smartphone dual-mode detection of dopamine based on nitrogen-boron co-doped carbon quantum dots
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-15 , DOI: 10.1007/s00604-020-04543-w
Sahar Dadkhah ₁ , Ali Mehdinia ₂ , Ali Jabbari ₁ , Ahmad Manbohi ₂

Affiliation

A dual-mode fluorescence and colorimetric biosensor based on nitrogen-boron co-doped carbon quantum dot (N-B CQDs) for rapid and sensitive detection of dopamine (DA) was developed. The quantum dot luminescent materials, N-B CQDs, were prepared by a one-step microwave-assisted method. The N-B CQDs were characterized using SEM, HR-TEM, XRD, FT-IR, Raman, fluorescence, and UV-Vis techniques. The dual-mode assays of fluorescence and colorimetric methods were used for detection of DA. The high fluorescent N-B CQDs mediated turn-off assay for the facile room temperature detection of dopamine via inner filter effect (IFE) and Forster resonance energy transfer (FRET) processes at basic pH. The colorimetric detection of DA was also developed via in-house android application using a smartphone and N-B CQD solution-based nanosensor. The smartphone-based colorimetric biosensors generated more reliable information for quantitative analysis of color changes than the naked eye. Furthermore, a smartphone application with N-B CQD solution-based nanosensor was integrated to monitor the color changes through the DA addition. Wide linear ranges were achieved for DA in the ranges 0.25–50 μM and 5–500 μM with fluorescence and smartphone-based method, respectively. The satisfactory results of the dual-mode detection of DA, not only in aqueous solution, but also in human urine and serum biological sample demonstrated its potential application in biosensing, as a point of care diagnostic tool. Graphical abstract Graphical abstract

中文翻译：

基于氮硼共掺杂碳量子点的多巴胺快速灵敏荧光和智能手机双模检测

开发了一种基于氮硼共掺杂碳量子点 (NB CQD) 的双模式荧光比色生物传感器，用于快速灵敏地检测多巴胺 (DA)。量子点发光材料NB CQDs是通过一步微波辅助方法制备的。使用 SEM、HR-TEM、XRD、FT-IR、拉曼、荧光和 UV-Vis 技术对 NB CQD 进行表征。荧光和比色法的双模式测定用于检测DA。高荧光 NB CQDs 介导的关闭测定，用于在基本 pH 下通过内部过滤效应 (IFE) 和 Forster 共振能量转移 (FRET) 过程在室温下轻松检测多巴胺。DA 的比色检测也是通过内部 android 应用程序使用智能手机和基于 NB CQD 解决方案的纳米传感器开发的。基于智能手机的比色生物传感器为颜色变化的定量分析生成了比肉眼更可靠的信息。此外，集成了基于 NB CQD 解决方案的纳米传感器的智能手机应用程序，以通过添加 DA 来监测颜色变化。使用荧光和基于智能手机的方法分别在 0.25-50 μM 和 5-500 μM 范围内实现了 DA 的宽线性范围。DA 双模式检测的令人满意的结果，不仅在水溶液中，而且在人尿和血清生物样品中也证明了其在生物传感中的潜在应用，作为即时诊断工具。图形摘要图形摘要集成了基于 NB CQD 解决方案的纳米传感器的智能手机应用程序，以通过添加 DA 来监控颜色变化。使用荧光和基于智能手机的方法分别在 0.25-50 μM 和 5-500 μM 范围内实现了 DA 的宽线性范围。DA 双模式检测的令人满意的结果，不仅在水溶液中，而且在人尿和血清生物样品中也证明了其在生物传感中的潜在应用，作为即时诊断工具。图形摘要图形摘要集成了基于 NB CQD 解决方案的纳米传感器的智能手机应用程序，以通过添加 DA 来监控颜色变化。使用荧光和基于智能手机的方法分别在 0.25-50 μM 和 5-500 μM 范围内实现了 DA 的宽线性范围。DA 双模式检测的令人满意的结果，不仅在水溶液中，而且在人尿和血清生物样品中也证明了其在生物传感中的潜在应用，作为即时诊断工具。图形摘要图形摘要不仅在水溶液中，而且在人尿和血清生物样本中也证明了其在生物传感中的潜在应用，作为即时诊断工具。图形摘要图形摘要不仅在水溶液中，而且在人尿和血清生物样本中也证明了其在生物传感中的潜在应用，作为即时诊断工具。图形摘要图形摘要

更新日期：2020-09-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11