Abstract

A number of models of the polymerase chain reaction have been suggested to date with the aim of gaining exact quantitative estimates for reaction products. These models usually identify the reaction product kinetics with a kinetics of fluorescence reporter. Here, a model of polymerase chain reaction is suggested in which the results of the reaction are detected using an intercalating dye. Simulation data indicate a significant discrepancy between the reaction product kinetics and the fluorescent signal from a bonded intercalator.

中文翻译：

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插入染料在聚合酶链反应过程中产生荧光信号的模型

摘要

迄今为止，已经提出了许多聚合酶链反应模型，目的是获得反应产物的精确定量估计。这些模型通常用荧光报告分子的动力学来鉴定反应产物的动力学。在此，提出了聚合酶链式反应的模型，其中使用嵌入染料检测反应的结果。模拟数据表明反应产物动力学与键合嵌入剂发出的荧光信号之间存在显着差异。