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Model of Fluorescent Signal Generation from an Intercalating Dye in the Course of a Polymerase Chain Reaction

  • PHYSICAL METHODS IN PHOTOBIOLOGY
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Abstract

A number of models of the polymerase chain reaction have been suggested to date with the aim of gaining exact quantitative estimates for reaction products. These models usually identify the reaction product kinetics with a kinetics of fluorescence reporter. Here, a model of polymerase chain reaction is suggested in which the results of the reaction are detected using an intercalating dye. Simulation data indicate a significant discrepancy between the reaction product kinetics and the fluorescent signal from a bonded intercalator.

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Funding

This study was carried out in the framework of state order no. 075-00780-19-02 of the Ministry of Science and Higher Education of the Russian Federation, grant no. 0074-2019-0013.

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Authors and Affiliations

  1. Institute of Analytical Instrumentation, Russian Academy of Sciences, 190103, St. Petersburg, Russia

    A. A. Fedorov

  2. ZAO Sintol, 127550, Moscow, Russia

    D. G. Sochivko & D. A. Varlamov

Authors
  1. A. A. Fedorov
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  2. D. G. Sochivko
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  3. D. A. Varlamov
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Correspondence to A. A. Fedorov.

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The authors declare that they have no conflicts of interest.

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Translated by V. Isaakyan

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Fedorov, A.A., Sochivko, D.G. & Varlamov, D.A. Model of Fluorescent Signal Generation from an Intercalating Dye in the Course of a Polymerase Chain Reaction. Tech. Phys. 65, 1516–1520 (2020). https://doi.org/10.1134/S1063784220090169

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  • DOI: https://doi.org/10.1134/S1063784220090169

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