Aberrant expression of mTOR signaling pathway is significantly associated with gastric cancer. However, the effect of smoking on mTOR expression and its downstream signaling molecules in gastric cancer has not been explored. Our study aims to investigate the effect of smoking on p-mTOR and its correlation with various downstream targets and survival of the smoker and never-smoker in advanced gastric cancer patients. Forty-one smokers and 41 never-smokers patient sample with the advanced gastric carcinoma were chosen for this study. Immunohistochemistry and western blot analysis were performed to check the expression of p-mTOR and its downstream targets. The correlation of p-mTOR with its downstream targets was analyzed by linear regression analysis in Graph Pad Prism software. Survivability analysis was examined by Kaplan–Meier method with log rank test in SPSS. High expression of p-mTOR and its downstream targets were observed in advanced gastric cancer smoker patients as compared to never-smokers by immunohistochemistry and western blot analysis. Results revealed that over expressed p-mTOR in smoker patients were positively correlated with its downstream targets (P < 0.05) and poor survival (P = 0.034). Over expression of p-mTOR in gastric cancer male smoker patients had the worse outcome.

mTOR信号通路的异常表达与胃癌显着相关。然而，尚未研究吸烟对胃癌中mTOR表达及其下游信号分子的影响。我们的研究旨在调查吸烟对p-mTOR的影响及其与各种下游靶标的关系以及晚期胃癌患者吸烟者和永不吸烟者的生存率。本研究选择了41位吸烟者和41位从不吸烟的晚期胃癌患者样本。进行了免疫组织化学和蛋白质印迹分析以检查p-mTOR及其下游靶标的表达。通过Graph Pad Prism软件中的线性回归分析来分析p-mTOR与下游目标的相关性。通过SPSS中的log rank检验，通过Kaplan–Meier方法检查生存能力分析。通过免疫组织化学和蛋白质印迹分析，与从未吸烟者相比，晚期胃癌吸烟者患者中观察到了p-mTOR及其下游靶标的高表达。结果显示，吸烟患者中过度表达的p-mTOR与其下游目标呈正相关（P <0.05），且生存期较差（P = 0.034）。在胃癌男性吸烟者中，p-mTOR的过表达具有更差的结果。0.05）和较差的生存率（P = 0.034）。在胃癌男性吸烟者中，p-mTOR的过表达具有更差的结果。0.05）和较差的生存率（P = 0.034）。在胃癌男性吸烟者中，p-mTOR的过表达具有更差的结果。