Abstract
Aberrant expression of mTOR signaling pathway is significantly associated with gastric cancer. However, the effect of smoking on mTOR expression and its downstream signaling molecules in gastric cancer has not been explored. Our study aims to investigate the effect of smoking on p-mTOR and its correlation with various downstream targets and survival of the smoker and never-smoker in advanced gastric cancer patients. Forty-one smokers and 41 never-smokers patient sample with the advanced gastric carcinoma were chosen for this study. Immunohistochemistry and western blot analysis were performed to check the expression of p-mTOR and its downstream targets. The correlation of p-mTOR with its downstream targets was analyzed by linear regression analysis in Graph Pad Prism software. Survivability analysis was examined by Kaplan–Meier method with log rank test in SPSS. High expression of p-mTOR and its downstream targets were observed in advanced gastric cancer smoker patients as compared to never-smokers by immunohistochemistry and western blot analysis. Results revealed that over expressed p-mTOR in smoker patients were positively correlated with its downstream targets (P < 0.05) and poor survival (P = 0.034). Over expression of p-mTOR in gastric cancer male smoker patients had the worse outcome.
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The data sets or information generated and analyzed during the present study are available in the CNCI repository. The datasets can be available from the corresponding author on reasonable requests.
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Acknowledgements
This study was supported in part by the Chittaranjan National Cancer Institute, Kolkata, India and by University Grants commission of India. Nabendu Murmu sincerely thanks to Dr. Jayanta Chakrabarti, Director, Chittaranjan National Cancer Institute (CNCI), Kolkata, India for his active support.
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This project is funded by University Grant Commission (UGC) Ref. No.: 17 − 06/2012(i)EU-V.
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PG and NM are responsible for the conception or design of the work. NA helped in providing the tissue samples of gastric cancer patients required for the project. PG and SM performed the acquisition, analysis, or interpretation of data of the work. SMM helped in reviewing the histopathology data of this project and provided the data. All authors finally approved the manuscript version to be published. Nabendu Murmu is the guarantor of the article. All authors read and approved the final manuscript.
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The study was approved by the Institutional Ethics Committee (IEC) of Chittaranjan National Cancer Institute, Kolkata, India (IEC Ref: A-4.311/1/2018-2).
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Consent was obtained from all patients included within the study. All the procedures of the present study were carried out in accordance with the Helsinki declaration and its later amendments.
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Ghosh, P., Alam, N., Mandal, S. et al. Association of mTOR pathway with risk of gastric cancer in male smoker with potential prognostic significance. Mol Biol Rep 47, 7489–7495 (2020). https://doi.org/10.1007/s11033-020-05808-6
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DOI: https://doi.org/10.1007/s11033-020-05808-6