Developing artificial enzyme mimetics for the detection of small biomolecules are a current research interest because natural enzymes bear some serious disadvantages, such as their catalytic activity can be easily inhibited and they can be digested by proteases. Herein, a heteropoly acids (HPA) encapsulating metal–organic framework (MOF) with metal-carbene structure, [Cu₁₀(H₃trz)₄(Htrz)₄][PMo₁₂VO₄₁] (PMA-MOF) as bifunctional enzyme-mimetic catalyst for colorimetric detection of hydrogen peroxide (H₂O₂) and ascorbic acid (AA) was designed and synthesized. Thanks to the good stability and the synergistic effect of PMA and MOF, PMA-MOF exhibits the lower limit of detection (0.222 μM towards H₂O₂ and 0.0046 μM to AA), and the smaller Km value (0.0138 mM for H₂O₂ and 0.136 mM for o-phenylenediamine) compared to most reported MOF- and HPA-based enzyme-mimetic catalyst, to the best our knowledge.

开发用于检测小生物分子的人造酶模拟物是当前的研究兴趣，因为天然酶具有一些严重的缺点，例如它们的催化活性很容易被抑制并且可以被蛋白酶消化。在此，杂多酸（HPA）封装了具有金属碳烯结构的金属有机骨架（MOF），[Cu ₁₀（H ₃ trz）₄（Htrz）₄ ] [PMo ₁₂ VO ₄₁ ]（PMA-MOF）作为双功能酶比色法检测过氧化氢（H ₂ O ₂）和抗坏血酸（AA）的设计和合成。多亏了良好的稳定性和PMA和MOF的协同效应，PMA-MOF显示出检测的（0.222下限 μ朝向高中₂ ö ₂和0.0046  μ M至AA），并且较小的Km值（0.0138毫用于h据我们所知，与大多数报道的基于MOF和HPA的酶模拟催化剂相比，邻苯二胺的浓度为₂ O ₂和0.136 mM。