Abstract

To estimate the size of nanostructures in biological fluids and study the dynamics of their change, a modified method of laser correlation spectroscopy is proposed. The scheme of the hardware-software complex and the algorithm of the method are described, which allows one to achieve high accuracy in determining the size of nanoparticles, as well as to study the process of changing the size of nanoparticles in dynamics. The proposed hardware-software complex made it possible to study the dynamics of the formation of aggregates in human serum in the process of immune response. The results obtained indicate the presence of processes of rapid protein aggregation as a result of activation of the immune response. In addition, the size of the aggregates formed depends on the state of the immune system and the presence of diseases.

中文翻译：

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用于评估生物流体中结构的尺寸和尺寸变化动力学的激光相关光谱仪

摘要

为了估计生物流体中纳米结构的尺寸并研究其变化的动力学，提出了一种改进的激光相关光谱法。描述了硬件-软件复合体的方案和该方法的算法，其使得可以在确定纳米颗粒的尺寸方面实现高精度，并且可以研究动力学中改变纳米颗粒的尺寸的过程。所提出的硬件-软件复合体使得研究免疫应答过程中人血清中聚集体形成的动力学成为可能。所获得的结果表明，由于免疫应答的激活，存在快速蛋白质聚集的过程。另外，形成的聚集体的大小取决于免疫系统的状态和疾病的存在。