The present study was conducted to determine efficiency of green tissue-specific (pRCA) and stress-inducible promoters (pRD29A) to express E. coli beta-glucuronidase (gusA) gene in transgenic potatoes compared with constitutive promoter (35S CaMV). The promoter fragments were isolated from their original source and cloned upstream to gusA in pCAMBIA-1301 binary vector to develop plant expression constructs, i.e., pRCA-pCAMBIA and pRD29A-pCAMBIA. Agrobacterium strain GV2260 harboring recombinant plasmids were used to infect leaf discs and internodal explant of Lady Olympia cultivar. GUS histochemical analysis was performed at different stages to determine GUS activity in transgenic plants. To determine activity of stress-inducible promoter (pRD29A), transgenic plants were exposed to heat, drought and combination of both heat and drought stress. The real time (RT-qPCR) and GUS florimetric assays revealed that pRD29A promoter gets more activated under drought, heat and combination of both stresses. GUS expression levels were more than 10 folds high with pRD29A promoter compared to control. Likewise, the reduced transcripts levels of gusA gene under control of pRCA promoter were found in tuber/roots of transgenic plants compared to 35S promoter. GUS florimetric assays also showed decreased or no GUS expression in tubers. In conclusion, the results encourage the appropriate use of promoters to drive the expression of foreign gene(s) for the development of potato lines tolerant to biotic and abiotic stress while minimizing the risks of transgenic technology in potatoes.

本研究旨在确定与组成型启动子 (35S CaMV) 相比，绿色组织特异性 (pRCA) 和应激诱导型启动子 (pRD29A) 在转基因马铃薯中表达大肠杆菌β-葡萄糖醛酸酶 ( gusA ) 基因的效率。从其原始来源分离启动子片段并克隆至pCAMBIA-1301双元载体中的gusA上游以开发植物表达构建体，即pRCA-pCAMBIA和pRD29A-pCAMBIA。使用携带重组质粒的农杆菌菌株 GV2260 感染 Lady Olympia 品种的叶盘和节间外植体。在不同阶段进行GUS组织化学分析以确定转基因植物中的GUS活性。为了确定胁迫诱导启动子（pRD29A）的活性，将转基因植物暴露于热、干旱以及热和干旱胁迫的组合。实时 (RT-qPCR) 和 GUS 花色测定表明，pRD29A 启动子在干旱、高温和两种胁迫的组合下变得更加激活。与对照相比，pRD29A启动子的GUS表达水平高10倍以上。同样，与35S启动子相比，在转基因植物的块茎/根中发现在pRCA启动子控制下的gusA基因的转录物水平降低。 GUS 花色测定也显示块茎中 GUS 表达减少或没有。总之，这些结果鼓励适当使用启动子来驱动外源基因的表达，以开发耐受生物和非生物胁迫的马铃薯品系，同时最大限度地降低马铃薯转基因技术的风险。