Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells' metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 - myoblasts, and B16-F10 - melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.

中文翻译：

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小鼠细胞系活细胞和死细胞自体荧光水平的变化。

细胞亚群的无标记表征是一种非常有前途的生物医学方法。如今，有几种基于不同物理特性（如大小、密度、刚度等）的无标签方法，可以对生物对象进行表征。然而，荧光特性是组织和细胞的无标记研究最合适的特征。了解正常和病理/活细胞和死细胞的自发荧光水平特性可以成为细胞代谢活动、活力评估和许多疾病诊断的有用工具。在这项研究中，我们应用了一系列小鼠细胞系（RAW 264.7 - 巨噬细胞，L929 - 成纤维细胞，C2C12 - 成肌细胞，