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Changes in Autofluorescence Level of Live and Dead Cells for Mouse Cell Lines

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Abstract

Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells’ metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 – myoblasts, and B16-F10 – melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.

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Funding

This research was funded by the Russian Science Foundation grant number 18–19-00354.

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Authors and Affiliations

  1. Saratov State University, Saratov, Russia

    Anastasiia A. Kozlova, Roman A. Verkhovskii, Alexey V. Ermakov & Daniil N. Bratashov

  2. Yuri Gagarin State Technical University of Saratov, Saratov, Russia

    Roman A. Verkhovskii

  3. I. M. Sechenov First Moscow State Medical University, Moscow, Russia

    Alexey V. Ermakov

Authors
  1. Anastasiia A. Kozlova
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  2. Roman A. Verkhovskii
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  3. Alexey V. Ermakov
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  4. Daniil N. Bratashov
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Correspondence to Anastasiia A. Kozlova.

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Kozlova, A.A., Verkhovskii, R.A., Ermakov, A.V. et al. Changes in Autofluorescence Level of Live and Dead Cells for Mouse Cell Lines. J Fluoresc 30, 1483–1489 (2020). https://doi.org/10.1007/s10895-020-02611-1

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  • DOI: https://doi.org/10.1007/s10895-020-02611-1

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