A novel cytorhabdovirus, tentatively named “paper mulberry mosaic-associated virus” (PMuMaV), was discovered and identified by transcriptome sequencing (RNA-seq), small RNA sequencing (sRNA-seq), and RT-PCR amplification. The whole-genome sequence of PMuMaV is 13,736 nucleotides (nt) in length and contains six open reading frames (ORFs) encoding a nucleocapsid protein (N), a phosphoprotein (P), a putative movement protein (P3), a matrix protein (M), a glycoprotein (G), and an RNA-dependent RNA polymerase (L). The coding sequences are flanked by a 194-nt leader and a 370-nt trailer sequence at the 3’ terminus and 5’ terminus, respectively. Pairwise sequence comparisons showed that PMuMaV is related to northern cereal mosaic virus (NCMV, 38.97%), barley yellow striate mosaic virus (BYSMV, 38.86%), and maize yellow striate virus (MYSV, 38.76%), and phylogenetic analysis also placed these viruses together into the same branch, thus suggesting that PMuMaV is a member of a new species in the genus Cytorhabdovirus.

通过转录组测序（RNA-seq），小RNA测序（sRNA-seq）和RT-PCR扩增，发现并鉴定了一种新型的细胞病毒，暂定名为“纸桑花叶病相关病毒”（PMuMaV）。PMuMaV的全基因组序列长度为13,736个核苷酸（nt），包含六个开放阅读框（ORF），它们编码核衣壳蛋白（N），磷酸蛋白（P），推定运动蛋白（P3），基质蛋白（ M），糖蛋白（G）和RNA依赖性RNA聚合酶（L）。编码序列在3'末端和5'末端分别带有194-nt前导序列和370-nt尾随序列。成对序列比较显示PMuMaV与北部谷物花叶病毒（NCMV，38.97％），大麦黄色条纹花叶病毒（BYSMV，38.86％）和玉米黄色条纹病毒（MYSV，38.76％）有关，杆状病毒。