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Molecular characterization of a novel cytorhabdovirus associated with paper mulberry mosaic disease.
Archives of Virology ( IF 2.5 ) Pub Date : 2020-08-26 , DOI: 10.1007/s00705-020-04786-1
Yuanjian Qiu 1, 2 , Song Zhang 1, 2 , Jingjing Jin 1, 2 , Jiaxi Xie 1, 2 , Yumei Cao 1, 2 , Mengji Cao 1, 2
Affiliation  

A novel cytorhabdovirus, tentatively named “paper mulberry mosaic-associated virus” (PMuMaV), was discovered and identified by transcriptome sequencing (RNA-seq), small RNA sequencing (sRNA-seq), and RT-PCR amplification. The whole-genome sequence of PMuMaV is 13,736 nucleotides (nt) in length and contains six open reading frames (ORFs) encoding a nucleocapsid protein (N), a phosphoprotein (P), a putative movement protein (P3), a matrix protein (M), a glycoprotein (G), and an RNA-dependent RNA polymerase (L). The coding sequences are flanked by a 194-nt leader and a 370-nt trailer sequence at the 3’ terminus and 5’ terminus, respectively. Pairwise sequence comparisons showed that PMuMaV is related to northern cereal mosaic virus (NCMV, 38.97%), barley yellow striate mosaic virus (BYSMV, 38.86%), and maize yellow striate virus (MYSV, 38.76%), and phylogenetic analysis also placed these viruses together into the same branch, thus suggesting that PMuMaV is a member of a new species in the genus Cytorhabdovirus.



中文翻译:

与纸桑叶花叶病有关的新型细胞病毒的分子表征。

通过转录组测序(RNA-seq),小RNA测序(sRNA-seq)和RT-PCR扩增,发现并鉴定了一种新型的细胞病毒,暂定名为“纸桑花叶病相关病毒”(PMuMaV)。PMuMaV的全基因组序列长度为13,736个核苷酸(nt),包含六个开放阅读框(ORF),它们编码核衣壳蛋白(N),磷酸蛋白(P),推定运动蛋白(P3),基质蛋白( M),糖蛋白(G)和RNA依赖性RNA聚合酶(L)。编码序列在3'末端和5'末端分别带有194-nt前导序列和370-nt尾随序列。成对序列比较显示PMuMaV与北部谷物花叶病毒(NCMV,38.97%),大麦黄色条纹花叶病毒(BYSMV,38.86%)和玉米黄色条纹病毒(MYSV,38.76%)有关,杆状病毒

更新日期:2020-10-11
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