Skip to main content
SpringerLink
Log in

Molecular characterization of a novel cytorhabdovirus associated with paper mulberry mosaic disease

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A novel cytorhabdovirus, tentatively named “paper mulberry mosaic-associated virus” (PMuMaV), was discovered and identified by transcriptome sequencing (RNA-seq), small RNA sequencing (sRNA-seq), and RT-PCR amplification. The whole-genome sequence of PMuMaV is 13,736 nucleotides (nt) in length and contains six open reading frames (ORFs) encoding a nucleocapsid protein (N), a phosphoprotein (P), a putative movement protein (P3), a matrix protein (M), a glycoprotein (G), and an RNA-dependent RNA polymerase (L). The coding sequences are flanked by a 194-nt leader and a 370-nt trailer sequence at the 3’ terminus and 5’ terminus, respectively. Pairwise sequence comparisons showed that PMuMaV is related to northern cereal mosaic virus (NCMV, 38.97%), barley yellow striate mosaic virus (BYSMV, 38.86%), and maize yellow striate virus (MYSV, 38.76%), and phylogenetic analysis also placed these viruses together into the same branch, thus suggesting that PMuMaV is a member of a new species in the genus Cytorhabdovirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Reference

  1. Kuzmin I, Novella I, Dietzgen R, Padhi A, Rupprecht C (2009) The rhabdoviruses: biodiversity, phylogenetics, and evolution. Infect Genet Evol 9:541–553

    Article  CAS  Google Scholar 

  2. Walker PJ, Blasdell KR, Calisher CH, Dietzgen RG, Kondo H, Kurath G, Longdon B, Stone DM, Tesh RB, Tordo N, Vasilakis N, Whitfield AE (2018) ICTV virus taxonomy profile: Rhabdoviridae. J Gen Virol 99:447–448

    Article  CAS  Google Scholar 

  3. Dietzgen RG, Kondo H, Goodin MM, Kurath G, Vasilakis N (2017) The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins. Virus Res 227:158–170

    Article  CAS  Google Scholar 

  4. Mann KS, Dietzgen RG (2014) Plant rhabdoviruses: new insights and research needs in the interplay of negative-strand RNA viruses with plant and insect hosts. Arch Virol 159:1889–1900

    Article  CAS  Google Scholar 

  5. Whitfield AE, Huot OB, Martin KM, Kondo H, Dietzgen RG (2018) Plant rhabdoviruses: their origins and vector interactions. Cur Opin Virol 33:198–207

    Article  CAS  Google Scholar 

  6. Chang CS, Liu HL, Moncada X, Seelenfreund A, Seelenfreund D, Chung KF (2015) A holistic picture of Austronesian migrations revealed by phylogeography of Pacific paper mulberry. Proc Natl Acad Sci USA 112:13537–13542

    Article  CAS  Google Scholar 

  7. Peng X, Liu H, Chen P, Tang F, Hu Y, Wang F, Pi Z, Zhao M, Chen N, Chen H, Zhang X, Yan X, Liu M, Fu X, Zhao G, Yao P, Wang L, Dai H, Li X, Xiong W, Xu W, Zheng H, Yu H, Shen S (2019) A chromosome-scale genome assembly of paper mulberry (Broussonetia papyrifera) provides new insights into its forage and papermaking usage. Mol Plant 12:661–677

    Article  CAS  Google Scholar 

  8. Saito K, Linquist B, Keobualapha B, Shiraiwa T, Horie T (2009) Broussonetia papyrifera (paper mulberry): its growth, yield and potential as a fallow crop in slash-and-burn upland rice system of northern Laos. Agrofor Syst 76:525–532

    Article  Google Scholar 

  9. Liu QZ, Wu TQ, Davis RE, Zhao Y (2004) First report of witches’ broom disease of Broussonetia papyrifera and its association with a phytoplasma of elm yellows group (16SrV). Plant Dis 91:227

    Google Scholar 

  10. Wu PS, Chen K, Du HZ, Yan J, Zhang QE (2011) First report of Cochliobolus sativus causing leaf spot on paper mulberry in China. Plant Dis 95:1586

    Article  CAS  Google Scholar 

  11. Chiou HC, Chen TH, Chang CA, Tzeng J (2014) Characterization of a carlavirus associated with paper mulberry mosaic disease in Taiwan. Plant Prot Bull (Taipei) 56:1–24

    CAS  Google Scholar 

  12. Qiu Y, Zhang S, Yu H, Xuan Z, Yang L, Zhan B, Zerbini FM, Cao M (2020) Identification and characterization of two novel geminiviruses associated with paper mulberry (Broussonetia papyrifera) leaf curling disease. Plant Dis. https://doi.org/10.1094/PDIS-12-19-2597-RE

    Article  Google Scholar 

  13. Cao M, Zhang S, Li M, Liu Y, Dong P, Li S, Kuang M, Li R, Zhou Y (2019) Discovery of four novel viruses associated with flower yellowing disease of green Sichuan Pepper (Zanthoxylum Armatum) by virome analysis. Viruses 11:696

    Article  CAS  Google Scholar 

  14. Wang X, Wu Q, Itoa T, Cilloc F, Li W, Chen X, Yu J, Ding S (2010) RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in Arabidopsis thaliana. Proc Natl Acad Sci USA 107:484–489

    Article  CAS  Google Scholar 

  15. Bejerman N, Dietzgen R (2019) Letter to the editor: bean-associated cytorhabdovirus and papaya cytorhabdovirus are strains of the same virus. Viruses 11:230

    Article  CAS  Google Scholar 

  16. Bourhy H, Cowley JA, Larrous F, Holmes EC, Walker PJ (2005) Phylogenetic relationships among rhabdoviruses inferred using the L polymerase gene. J Gen Virol 86:2849–2858

    Article  CAS  Google Scholar 

  17. Liu Y, Du Z, Wang H, Zhang S, Cao M, Wang X (2018) Identification and characterization of wheat yellow striate virus, a novel leafhopper-transmitted nucleorhabdovirus infecting wheat. Front Microbiol 9:468

    Article  Google Scholar 

  18. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis 9 version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Key R&D Program of China (2019YFD1001800), Fundamental Research Funds for the Central Universities (XDJK2018AA002), Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjBX0016), Innovation Program for Chongqing’s Overseas Returnees (cx2019013), Overseas Expertise Introduction Project for Discipline Innovation (111 Project) (B18044), and Chongqing Postgraduate Research Innovation Project (CYS20135). We thank the three anonymous reviewers and Dr. Pedro Luis Ramos-González for their constructive comments and suggestions.

Author information

Author notes

  1. Yuanjian Qiu and Song Zhang contributed equally to this work.

Authors and Affiliations

  1. National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing, China

    Yuanjian Qiu, Song Zhang, Jingjing Jin, Jiaxi Xie, Yumei Cao & Mengji Cao

  2. State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China

    Yuanjian Qiu, Song Zhang, Jingjing Jin, Jiaxi Xie, Yumei Cao & Mengji Cao

Authors
  1. Yuanjian Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Song Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jingjing Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiaxi Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yumei Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mengji Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mengji Cao.

Ethics declarations

Conflict of interest

All authors declare they have no conflict of interest.

Ethical standards

I have read and abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Stephen John Wylie.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 269 kb)

Supplementary material 2 (DOCX 24 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qiu, Y., Zhang, S., Jin, J. et al. Molecular characterization of a novel cytorhabdovirus associated with paper mulberry mosaic disease. Arch Virol 165, 2703–2707 (2020). https://doi.org/10.1007/s00705-020-04786-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-020-04786-1

Search

Navigation