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Scale-up of a Fibonacci-Type Photobioreactor for the Production of Dunaliella salina.
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2020-08-26 , DOI: 10.1007/s12010-020-03410-x
Juan Pablo Díaz 1, 2 , Cristian Inostroza 2 , Francisco Gabriel Acién 2
Affiliation  

In this work, the previously proposed Fibonacci-type photobioreactor is scaled up and evaluated to produce Dunaliella salina. First, the composition of the culture medium was optimized to achieve maximal productivity. Next, the Fibonacci-type reactor was scaled up to 1250 L maintaining high solar radiation interception capacity of this type of reactor. Finally, the performance of the reactor for the production of green cells of Dunaliella salina at the environmental conditions prevailing in the Atacama Desert was evaluated. Data demonstrated that the proposed photobioreactor allows the temperature, pH and dissolved oxygen concentration to be maintained within the optimal ranges recommended for the selected strain. Both better exposure to solar radiation and photonic flow dilution avoids the use of cooling systems to prevent overheating under outdoor conditions. The system allows up to 60% more solar radiation to be intercepted than does the horizontal surface, likewise, allowing to maintain the pH efficiently through CO2 injection and to keep the dissolved oxygen concentration in acceptable ranges, thanks to its adequate mass transfer capacity. The biomass concentration reached up to 0.96 g L-1, three times higher than that obtained in a raceway reactor under the same environmental conditions, whereas productivity was up to 0.12 g L-1 day (2.41 g m-2 day). Maximum specific outdoor growth rates reached up to 0.17 day-1. Undoubtedly, this technology scaled up constitutes a new type of photobioreactor for use at the industrial scale since it is capable of maximizing biomass productivity under high light conditions.

中文翻译:

用于生产杜氏盐藻的斐波那契型光生物反应器的规模放大。

在这项工作中,将先前提出的斐波那契型光生物反应器放大并评估以生产杜氏盐藻。首先,优化培养基的组成以实现最大的生产率。接下来,将斐波那契型反应器按比例放大至1250 L,以保持此类反应器的高太阳辐射拦截能力。最后,评估了在阿塔卡马沙漠盛行的环境条件下反应器生产杜氏盐藻绿色细胞的性能。数据表明,提出的光生物反应器可以将温度,pH和溶解氧浓度保持在为所选菌株推荐的最佳范围内。更好地暴露于太阳辐射和光子流稀释都避免了使用冷却系统来防止室外条件下的过热。与水平表面相比,该系统最多可拦截60%以上的太阳辐射,这归功于其足够的传质能力,可通过注入二氧化碳有效地保持pH值并将溶解氧浓度保持在可接受的范围内。在相同的环境条件下,生物质浓度达到0.96 g L-1,是在管道反应器中获得的三倍,而生产率高达0.12 g L-1天(2.41 g m-2天)。最大的特定室外增长率达到第1天的0.17。无疑,
更新日期:2020-08-26
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