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Scale-up of a Fibonacci-Type Photobioreactor for the Production of Dunaliella salina

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In this work, the previously proposed Fibonacci-type photobioreactor is scaled up and evaluated to produce Dunaliella salina. First, the composition of the culture medium was optimized to achieve maximal productivity. Next, the Fibonacci-type reactor was scaled up to 1250 L maintaining high solar radiation interception capacity of this type of reactor. Finally, the performance of the reactor for the production of green cells of Dunaliella salina at the environmental conditions prevailing in the Atacama Desert was evaluated. Data demonstrated that the proposed photobioreactor allows the temperature, pH and dissolved oxygen concentration to be maintained within the optimal ranges recommended for the selected strain. Both better exposure to solar radiation and photonic flow dilution avoids the use of cooling systems to prevent overheating under outdoor conditions. The system allows up to 60% more solar radiation to be intercepted than does the horizontal surface, likewise, allowing to maintain the pH efficiently through CO2 injection and to keep the dissolved oxygen concentration in acceptable ranges, thanks to its adequate mass transfer capacity. The biomass concentration reached up to 0.96 g L−1, three times higher than that obtained in a raceway reactor under the same environmental conditions, whereas productivity was up to 0.12 g L−1 day (2.41 g m−2 day). Maximum specific outdoor growth rates reached up to 0.17 day−1. Undoubtedly, this technology scaled up constitutes a new type of photobioreactor for use at the industrial scale since it is capable of maximizing biomass productivity under high light conditions.

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Acknowledgements

We wish to thank the members of the FONDEF project, D04I1258: Marcela Avila, Elizabet Rojas and Pablo Barria. The authors are also very grateful to Attilio Gattavara and Masatoshi Futagawa for their generous cooperation and especially to Claudio Brieba (R.I.P.) for being part of this dream.

Funding

This research was supported by EU H2020 SABANA project from the European Union’s Horizon 2020 Research and Innovation program under the Grant Agreement No. 727874.

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Authors and Affiliations

  1. Universidad Arturo Prat, Avda. Arturo Prat, 21-20, Iquique, Chile

    Juan Pablo Díaz

  2. Chemical Engineering Department, University of Almeria, Ctra. Sacramento, s/n, 04120, Almeria, Spain

    Juan Pablo Díaz, Cristian Inostroza & Francisco Gabriel Acién

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  1. Juan Pablo Díaz
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Díaz, J.P., Inostroza, C. & Acién, F.G. Scale-up of a Fibonacci-Type Photobioreactor for the Production of Dunaliella salina. Appl Biochem Biotechnol 193, 188–204 (2021). https://doi.org/10.1007/s12010-020-03410-x

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