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Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization.
Applied Biochemistry and Biotechnology ( IF 3.1 ) Pub Date : 2020-08-21 , DOI: 10.1007/s12010-020-03409-4
Hyeong Min Han 1 , In Jung Kim 1 , Eun Ju Yun 1 , Jae Won Lee 2, 3 , Yoonho Cho 1 , Yong-Su Jin 2, 3 , Kyoung Heon Kim 1
Affiliation  

Colanic acid (CA) is one of the major bacterial exopolysaccharides. Due to its biological activities, CA has a significant commercial value. However, the cultivation conditions have not been optimized for the large-scale production of CA. Here, we constructed a CA-overproducing Escherichia coli strain (ΔwaaF) and statistically optimized its culture media for maximum CA production. Glucose and tryptone were found the optimal carbon and nitrogen sources, respectively. Fractional factorial design indicated tryptone and Na2HPO4 as the critical nutrients for CA production. Through further optimization, we achieved a maximum CA production of 1910.0 mg/L, which is approximately 12-fold higher than the amount obtained using the non-optimized medium initially used. The predicted value of CA production was comparable with experimental value (2052.8 mg/L) under the optimized conditions. This study constitutes a successful demonstration of media optimization for increased CA production, and paves the way for future research for achieving large-scale CA production.



中文翻译:

通过菌株工程和培养基优化,大肠杆菌过量生产胞外多糖椰油酸。

钴酸(CA)是主要的细菌胞外多糖之一。由于其生物活性,CA具有重要的商业价值。但是,对于大规模生产CA,尚未优化培养条件。在这里,我们构建了一株过量生产CA的大肠杆菌ΔwaaF),并对其培养基进行了统计优化,以最大程度地生产CA。葡萄糖和胰蛋白were分别被发现是最佳的碳源和氮源。分数阶乘设计表明胰蛋白and和Na 2 HPO 4作为CA生产的关键营养素。通过进一步优化,我们实现了最高CA产量1910.0 mg / L,这比使用最初使用的非优化培养基获得的产量高约12倍。在最佳条件下,CA产量的预测值与实验值(2052.8 mg / L)相当。这项研究成功展示了如何通过优化媒体来提高CA的产量,并为将来实现大规模CA产量的研究铺平了道路。

更新日期:2020-08-21
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