The results of 3D culturing of human thyroid follicle-like structures in a gel based on platelet lysate at the gel-air interface are presented. During culturing up to 4 months, no new follicle-like structures were formed and none were destroyed. During the first 2 months, most follicle-like structures increased in size; then, their grown decelerated, but they retained viability. Ki-67 + cells were observed in the majority of follicle-like structures. Most of them produced thyroglobulin. Follicle-like structures get closer, the number of contacts between them increased, and cluster appeared. Thus, the developed 3D culturing system in a gel based on platelet lysate is an adequate approach for maintaining structure and functional activity of human follicle-like structures in vitro for at least 2 months.

中文翻译：

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基于血小板裂解物的凝胶中人类甲状腺滤泡样结构的初级 3D 培养

展示了在凝胶-空气界面处基于血小板裂解物的凝胶中人类甲状腺滤泡样结构的 3D 培养结果。在长达 4 个月的培养过程中，没有形成新的卵泡状结构，也没有被破坏。在前 2 个月内，大多数卵泡状结构的大小增加；然后，他们的成长减速，但他们保留了生存能力。在大多数毛囊样结构中观察到 Ki-67 + 细胞。他们中的大多数产生甲状腺球蛋白。毛囊状结构越来越近，它们之间的接触数量增加，出现簇状。因此，在基于血小板裂解物的凝胶中开发的 3D 培养系统是在体外维持人类卵泡样结构的结构和功能活性至少 2 个月的适当方法。