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Primary 3D Culture of Human Thyroid Follicle-Like Structures in Platelet Lysate-Based Gel
Bulletin of Experimental Biology and Medicine ( IF 0.9 ) Pub Date : 2020-08-01 , DOI: 10.1007/s10517-020-04930-6
N S Sergeeva 1, 2 , V A Kirsanova 1 , Yu D Khesuani 3 , I K Sviridova 1 , T E Skachkova 1 , V A Mironov 3 , A P Polyakov 1 , A D Kaprin 1
Affiliation  

The results of 3D culturing of human thyroid follicle-like structures in a gel based on platelet lysate at the gel-air interface are presented. During culturing up to 4 months, no new follicle-like structures were formed and none were destroyed. During the first 2 months, most follicle-like structures increased in size; then, their grown decelerated, but they retained viability. Ki-67 + cells were observed in the majority of follicle-like structures. Most of them produced thyroglobulin. Follicle-like structures get closer, the number of contacts between them increased, and cluster appeared. Thus, the developed 3D culturing system in a gel based on platelet lysate is an adequate approach for maintaining structure and functional activity of human follicle-like structures in vitro for at least 2 months.

中文翻译:

基于血小板裂解物的凝胶中人类甲状腺滤泡样结构的初级 3D 培养

展示了在凝胶-空气界面处基于血小板裂解物的凝胶中人类甲状腺滤泡样结构的 3D 培养结果。在长达 4 个月的培养过程中,没有形成新的卵泡状结构,也没有被破坏。在前 2 个月内,大多数卵泡状结构的大小增加;然后,他们的成长减速,但他们保留了生存能力。在大多数毛囊样结构中观察到 Ki-67 + 细胞。他们中的大多数产生甲状腺球蛋白。毛囊状结构越来越近,它们之间的接触数量增加,出现簇状。因此,在基于血小板裂解物的凝胶中开发的 3D 培养系统是在体外维持人类卵泡样结构的结构和功能活性至少 2 个月的适当方法。
更新日期:2020-08-01
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