The results of 3D culturing of human thyroid follicle-like structures in a gel based on platelet lysate at the gel-air interface are presented. During culturing up to 4 months, no new follicle-like structures were formed and none were destroyed. During the first 2 months, most follicle-like structures increased in size; then, their grown decelerated, but they retained viability. Ki-67+ cells were observed in the majority of follicle-like structures. Most of them produced thyroglobulin. Follicle-like structures get closer, the number of contacts between them increased, and cluster appeared. Thus, the developed 3D culturing system in a gel based on platelet lysate is an adequate approach for maintaining structure and functional activity of human follicle-like structures in vitro for at least 2 months.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 104-111, June, 2020
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Sergeeva, N.S., Kirsanova, V.A., Khesuani, Y.D. et al. Primary 3D Culture of Human Thyroid Follicle-Like Structures in Platelet Lysate-Based Gel. Bull Exp Biol Med 169, 564–570 (2020). https://doi.org/10.1007/s10517-020-04930-6
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DOI: https://doi.org/10.1007/s10517-020-04930-6