We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.

中文翻译：

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成骨条件培养基培养过程中RUNX2和Osterix在大鼠间充质干细胞中的表达

我们研究了在向间充质干细胞 (MSC) 的成骨分化培养基中加入成骨条件培养基浓缩液后转录因子 RUNX2 和 Osterix 的表达。获得的成骨条件培养基浓缩物含有分子量 > 10 kDa 的生物活性物质复合物，使 MSC 分化为成骨细胞，与阴性对照相比，转录因子 RUNX2 和 Osterix 的高水平表达证实了这一点。在存在成骨补充剂 StemPro（阳性对照）和研究的成骨条件培养基浓缩物的情况下，MSC 培养第 14 天显示转录因子 Osterix 的最高表达。